Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses
文献类型：期刊论文
| 作者 | Li, Kecheng1,2; Liu, Song1; Xing, Ronge1 ; Qin, Yukun1; Li, Pengcheng1
|
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2013-02-15 |
| 卷号 | 92期号:2页码:1730-1736 |
| 关键词 | Chitotriose N-acetylchitotriose N Separation Antioxidant activity N '-diacetylchitotriose |
| ISSN号 | 0144-8617 |
| 通讯作者 | Xing, RG |
| 中文摘要 | The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry ; Polymer Science |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | BIOLOGICAL-ACTIVITIES ; SCAVENGING ACTIVITY ; CHITIN DEACETYLASE ; IN-VITRO ; CHITOSAN ; CHITOOLIGOSACCHARIDES ; OLIGOSACCHARIDES ; OLIGOMERS ; CHITIN/CHITOSAN ; HYDROLYSIS |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.carbpol.2012.11.028 |
| 语种 | 英语 |
| WOS记录号 | WOS:000315616900099 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16714]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Liu, Song,Xing, Ronge,et al. Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses[J]. CARBOHYDRATE POLYMERS,2013,92(2):1730-1736. |
| APA | Li, Kecheng,Liu, Song,Xing, Ronge,Qin, Yukun,&Li, Pengcheng.(2013).Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses.CARBOHYDRATE POLYMERS,92(2),1730-1736. |
| MLA | Li, Kecheng,et al."Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses".CARBOHYDRATE POLYMERS 92.2(2013):1730-1736. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。