CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION
文献类型：期刊论文
| 作者 | Lin, Liang1,2; Xu, Qian1; Ma, Junchao1; Yuan, Bin1; Li, Weiqi1
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| 刊名 | CRYOLETTERS
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 35期号:2页码:129-137 |
| 关键词 | Orchidaceae Calanthe davidii sucrose preculture thin cell layer culture post-thawing osmo-conditioning |
| ISSN号 | 0143-2044 |
| 通讯作者 | Li,WQ (reprint author),Chinese Acad Sci,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Peoples R China. ; weiqili@mail.kib.ac.cn |
| 英文摘要 | BACKGROUND: Evaluation of the conservation Status of Calanthe plants in China indicated that 18 species were endangered or critically endangered. Comprehensive conservation solutions including ex situ methods, are urgently required to protect Calanthe species in China. OBJECTIVE: The study aims to develop a simple and efficient cryopreservation protocol using droplet-vitrification for Calanthe davidii. METHODS: Protocorm-like bodies (PLBs) were induced from nodal sections of in vitro shoots, and their proliferation was promoted by a thin cell layer culture procedure. Shoot tips excised from three leaf-stage PLBs were used in cryopreservation experiments. Key factors of the droplet-vitrification procedure including sucrose preculture, treatment with PVS2 solution and post-rewarming culture conditions were optimized to achieve a high level of regeneration. RESULTS: When the optimized procedure was applied, 77.8 +/- 3.9% of cryopreserved shoot tips withstood liquid nitrogen exposure and regenerated into new PLBs. CONCLUSION: These results highlighted the importance of post-rewarming osmo-conditioning for regeneration of cryopreserved shoot tips. |
| 类目[WOS] | Biology ; Physiology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics ; Physiology |
| 关键词[WOS] | THIN CELL-LAYERS ; HYBRID ORCHID BRATONIA ; TERRESTRIAL ORCHID ; BLETILLA-STRIATA ; IN-VITRO ; ENCAPSULATION-VITRIFICATION ; MATURE SEEDS ; REGENERATION ; CULTURE ; BODIES |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31070262];Fund of the State Key Laboratory of Phytocheinistry and Plant Resources in West China [0807B01211, 097C1211Z1];Kunming Institute of Botany [KSCX2-EW-J-24];Germplasm Bank of Wild Species;CAS Innovation Program of Kunming Institute [540806321211];100-Talents Program of CAS |
| 语种 | 英语 |
| WOS记录号 | WOS:000337718900008 |
| 公开日期 | 2014-07-28 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18223]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Liang,Xu, Qian,Ma, Junchao,et al. CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION[J]. CRYOLETTERS,2014,35(2):129-137. |
| APA | Lin, Liang,Xu, Qian,Ma, Junchao,Yuan, Bin,&Li, Weiqi.(2014).CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION.CRYOLETTERS,35(2),129-137. |
| MLA | Lin, Liang,et al."CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION".CRYOLETTERS 35.2(2014):129-137. |
入库方式： OAI收割
来源：昆明植物研究所
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