Characterization of Proapoptotic Compounds from the Bark of Garcinia oblongifolia
文献类型：期刊论文
| 作者 | Feng, Chao1; Huang, Sheng-Xiong2 ; Gao, Xue-Mei3,4; Xu, Hong-Xi5,6; Luo, Kathy Qian7
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| 刊名 | JOURNAL OF NATURAL PRODUCTS
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 77期号:5页码:1111-1116 |
| 关键词 | ANTICANCER DRUG SCREEN INHIBITS TUMOR-GROWTH HELA-CELLS APOPTOSIS ACTIVATION DEATH RESISTANCE DYNAMICS LINES HER2 |
| ISSN号 | 0163-3864 |
| 通讯作者 | Xu,HX (reprint author),Shanghai Univ Tradit Chinese Med,Sch Pharm,Shanghai 201203,Peoples R China. ; xuhongxi88@gmail.com ; kluo@ntu.edu.sg |
| 英文摘要 | Twenty compounds from Garcinia oblongifolia were screened for proapoptotic activity using FRET-based HeLa-C3 sensor cells. Among them, oblongifolins F and G (1 and 2), 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran[7,6-b]xanthone (3), nigrolineaxanthone T (4), and garcicowin B (5) showed significant proapoptotic activity at a concentration of 10 mu M. Bioassessments were then performed to evaluate the potential of these compounds for therapeutic application. All five compounds showed significant cytotoxicity and caspase-3-activating ability in cervical cancer He La cells, with compounds 1 and 2 having the highest potencies. All five compounds specifically induced caspase-dependent apoptosis, which could be prevented by the pan-caspase inhibitor zVAD-fmk. In particular, 3 induced apoptosis through mitotic arrest. Compounds 1-5 displayed similar IC50 values (3.9-16.5 mu M) against the three cancer cell lines He La, MDA-MB-435, and HepG2. In addition, compounds 1, 2, and 4 exhibited similar and potent IC50 values (2.4-5.1 mu M) against several breast and colon cancer cell lines, including those overexpressing either HER2 or P-glycoprotein. HER2 and P-glycoprotein are known factors that confer resistance to anticancer drugs in cancer cells. This is the first study on the cytotoxicity, caspase-3-activing ability, and specificity of proapoptotic compounds isolated from G. oblongifolia in He La cells. The potential application of these compounds against HER2- or P-glycoprotein-overexpressing cancer cells was investigated. |
| 学科主题 | Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 类目[WOS] | Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Pharmacology & Pharmacy |
| 关键词[WOS] | ANTICANCER DRUG SCREEN ; INHIBITS TUMOR-GROWTH ; HELA-CELLS ; APOPTOSIS ; ACTIVATION ; DEATH ; RESISTANCE ; DYNAMICS ; LINES ; HER2 |
| 收录类别 | SCI |
| 资助信息 | National Research Foundation of Singapore [NRF-CRP8-2011-05, M4092018.0S4];Ministry of Education of Singapore AcRF Tier 1 [M4010891.120];National Natural Science Foundation of China [21002085, 21362044] |
| 语种 | 英语 |
| WOS记录号 | WOS:000336510700003 |
| 公开日期 | 2014-07-28 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18184]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Clear Water Bay, Hong Kong, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Yunnan, Peoples R China 3.Yunnan Univ Nationalities, State Ethn Affairs Commiss, Key Lab Chem Ethn Med Resources, Kunming 650031, Yunnan, Peoples R China 4.Yunnan Univ Nationalities, Minist Educ, Kunming 650031, Yunnan, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China 6.Shanghai Coll TCM New Drug Discovery, Engn Res Ctr, Shanghai 201203, Peoples R China 7.Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, Singapore 637457, Singapore |
| 推荐引用方式 GB/T 7714 | Feng, Chao,Huang, Sheng-Xiong,Gao, Xue-Mei,et al. Characterization of Proapoptotic Compounds from the Bark of Garcinia oblongifolia[J]. JOURNAL OF NATURAL PRODUCTS,2014,77(5):1111-1116. |
| APA | Feng, Chao,Huang, Sheng-Xiong,Gao, Xue-Mei,Xu, Hong-Xi,&Luo, Kathy Qian.(2014).Characterization of Proapoptotic Compounds from the Bark of Garcinia oblongifolia.JOURNAL OF NATURAL PRODUCTS,77(5),1111-1116. |
| MLA | Feng, Chao,et al."Characterization of Proapoptotic Compounds from the Bark of Garcinia oblongifolia".JOURNAL OF NATURAL PRODUCTS 77.5(2014):1111-1116. |
入库方式： OAI收割
来源：昆明植物研究所
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