SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY
文献类型：期刊论文
| 作者 | Ping, Li1; Wang Yeling1; Guo Zhiyong1; Wu, Shibiao2,3; Kennelly, Edward J.1,2,3; Wang Jianjun1; Li, Tang1; Long Chunlin1,4 |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2014 |
| 卷号 | 37期号:18页码:2598-2609 |
| ISSN号 | 1082-6076 |
| 关键词 | Bidens pilosa L bioactive isomers countercurrent chromatography LC-TOF-MS methyl dicaffeoyl quinate separation |
| 通讯作者 | Long,CL (reprint author),Minzu Univ China,Coll Life & Environm Sci,27 Zhong Guan Cun South Ave,Beijing 100081,Peoples R China. ; long@mail.kib.ac.cn |
| 英文摘要 | Under LC-TOF-MS fraction-guided analysis, the n-butanol was extracted from Bidens pilosa. Three bioactivity isomers compounds, including methyl 3,4-dicaffeoyl quinate (1), methyl 3,5-dicaffeoyl quinate (2), and methyl 4,5-dicaffeoyl quinate (3) were successfully isolated by countercurrent chromatography (CCC) with two phase solvent system composed of chloroform-methanol-water-n-buthanol (4:3:2:0.5, v/v) elution. Their purities were 93.7%, 82.4%, and 91.8% determined by HPLC. HR-ESI-MS and NMR was used to confirm their structures. This is the first time the isolation of geometrical isomers of methyl dicaffeoyl quinates by CCC has been reported. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | METHYL 3,5-DICAFFEOYL QUINATE ; AERIAL PARTS ; PURIFICATION ; L. ; INVOLVEMENT ; DERIVATIVES ; EXTRACTS ; PLANTS ; CELLS |
| 资助信息 | National Natural Science Foundation of China [31070288, 31161140345];Ministry of Education of China [B08044, MUC 98506-01000101, MUC 98502-006004];Ministry of Science and Technology of China [2012FY110300-7];Fundamental Research Funds for the Central Universities [0910KYQN52] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337240500004 |
| 公开日期 | 2014-07-28 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18216]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China 2.CUNY Herbert H Lehman Coll, Dept Biol Sci, New York, NY USA 3.CUNY, Grad Ctr, New York, NY USA 4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ping, Li,Wang Yeling,Guo Zhiyong,et al. SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(18):2598-2609. |
| APA | Ping, Li.,Wang Yeling.,Guo Zhiyong.,Wu, Shibiao.,Kennelly, Edward J..,...&Long Chunlin.(2014).SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(18),2598-2609. |
| MLA | Ping, Li,et al."SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.18(2014):2598-2609. |
入库方式： OAI收割
来源：昆明植物研究所
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