MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development
文献类型：期刊论文
| 作者 | Xu, XH ; Deng, CY ; Liu, Y ; He, M ; Peng, J ; Wang, T ; Yuan, L ; Zheng, ZS ; Blackshear, PJ ; Luo, ZG |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 24期号:5页码:576-594 |
| 关键词 | PROTEIN-KINASE-C MAJOR SPECIFIC SUBSTRATE NEURONAL POLARIZATION PLASMA-MEMBRANE ANTEROGRADE TRANSPORT IN-VIVO PHOSPHORYLATION BRAIN GROWTH CELLS |
| ISSN号 | 1001-0602 |
| 通讯作者 | Luo, ZG (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,zgluo@ion.ac.cn |
| 英文摘要 | Axon development requires membrane addition from the intracellular supply, which has been shown to be mediated by Rab10-positive plasmalemmal precursor vesicles (PPVs). However, the molecular mechanisms underlying the membrane trafficking processes of PPVs remain unclear. Here, we show that myristoylated alanine-rich C-kinase substrate (MARCKS) mediates membrane targeting of Rab10-positive PPVs, and this regulation is critical for axon development. We found that the GTP-locked active form of Rab10 binds to membrane-associated MARCKS, whose affinity depends on the phosphorylation status of the MARCKS effector domain. Either genetic silencing of MARCKS or disruption of its interaction with Rab10 inhibited axon growth of cortical neurons, impaired docking and fusion of Rab10 vesicles with the plasma membrane, and consequently caused a loss of membrane insertion of axonal receptors responsive to extracellular axon growth factors. Thus, this study has identified a novel function of MARCKS in mediating membrane targeting of PPVs during axon development. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31330032, 31321091, 61327902, 81171190]; National Key Basic Research Program of China [2014CB910203]; SA-SIBS Scholarship Program; Shanghai Postdoctoral Scientific Program; China Postdoctoral Science Foundation; KC Wong Education Foundation |
| 语种 | 英语 |
| 公开日期 | 2014-07-30 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2608]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xu, XH,Deng, CY,Liu, Y,et al. MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development[J]. CELL RESEARCH,2014,24(5):576-594. |
| APA | Xu, XH.,Deng, CY.,Liu, Y.,He, M.,Peng, J.,...&Luo, ZG.(2014).MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.CELL RESEARCH,24(5),576-594. |
| MLA | Xu, XH,et al."MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development".CELL RESEARCH 24.5(2014):576-594. |
入库方式： OAI收割
来源：上海神经科学研究所
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