CaMKII alpha and caveolin-1 cooperate to drive ATP-induced membrane delivery of the P2X3 receptor
文献类型：期刊论文
| 作者 | Chen, XQ ; Zhu, JX ; Wang, Y ; Zhang, X ; Bao, L |
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:2页码:140-153 |
| 关键词 | PROTEIN-KINASE-II ROOT GANGLION NEURONS PRIMARY SENSORY NEURONS GENE-RELATED PEPTIDE D-ASPARTATE RECEPTOR P2X(3) RECEPTOR DIFFERENTIAL EXPRESSION NR2B SUBUNIT RAT PHOSPHORYLATION |
| ISSN号 | 1674-2788 |
| 通讯作者 | Bao, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,baolan@sibcb.ac.cn |
| 英文摘要 | The P2X3 receptor plays a vital role in sensory processing and transmission. The assembly and trafficking of the P2X3 receptor are important for its function in primary sensory neurons. As an important inflammation mediator, ATP is released from different cell types around primary sensory neurons, especially under pathological pain conditions. Here, we show that a, alpha,beta-MeATP dramatically promoted membrane delivery of the P2X3 receptor both in HEK293T cells expressing recombinant P2X3 receptor and in rat primary sensory neurons. alpha, beta-MeATP induced P2X3 receptor-mediated Ca2+ influx, which further activated Ca2+/calmodulin-dependent protein kinase II alpha (CaMKII alpha). The N terminus of the P2X3 receptor was responsible for CaMKIIa binding, whereas Thr(388) in the C terminus was phosphorylated by CaMKII alpha. Thr(388) phosphorylation increased P2X3 receptor binding to caveolin-1. Caveolin-1 knockdown abrogated the alpha, beta-MeATP-induced membrane insertion of the P2X3 receptor. Moreover, alpha,beta-MeATP drove the CaMKII alpha-mediated membrane coinsertion of the P2X2 receptor with the P2X3 receptor. The increased P2X3 receptors on the cell membrane that are due to Thr(388) phosphorylation facilitated P2X3 receptor-mediated signal transduction. Together, our data indicate that CaMKII alpha and caveolin-1 cooperate to drive ligand-induced membrane delivery of the P2X3 receptor and may provide a mechanism of P2X3 receptor sensitization in pain development. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30930044]; National Basic Research Program of China [2010CB912001, 2014CB942800] |
| 语种 | 英语 |
| 公开日期 | 2014-07-30 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2612]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Chen, XQ,Zhu, JX,Wang, Y,et al. CaMKII alpha and caveolin-1 cooperate to drive ATP-induced membrane delivery of the P2X3 receptor[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2014,6(2):140-153. |
| APA | Chen, XQ,Zhu, JX,Wang, Y,Zhang, X,&Bao, L.(2014).CaMKII alpha and caveolin-1 cooperate to drive ATP-induced membrane delivery of the P2X3 receptor.JOURNAL OF MOLECULAR CELL BIOLOGY,6(2),140-153. |
| MLA | Chen, XQ,et al."CaMKII alpha and caveolin-1 cooperate to drive ATP-induced membrane delivery of the P2X3 receptor".JOURNAL OF MOLECULAR CELL BIOLOGY 6.2(2014):140-153. |
入库方式： OAI收割
来源：上海神经科学研究所
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