Caspase-3 Cleavage of Dishevelled Induces Elimination of Postsynaptic Structures
文献类型：期刊论文
| 作者 | Wang, JY ; Chen, F ; Fu, XQ ; Ding, CS ; Zhou, L ; Zhang, XH ; Luo, ZG |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2014 |
| 卷号 | 28期号:6页码:670-684 |
| ISSN号 | 1534-5807 |
| 关键词 | NEUROMUSCULAR-JUNCTION SYNAPTIC PLASTICITY CELL-DEATH MAMMALIAN CASPASES RECEPTOR AGRIN ACTIVATION APOPTOSIS PROTEIN MUSCLE |
| 通讯作者 | Luo, ZG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zgluo@ion.ac.cn |
| 英文摘要 | During the development of vertebrate neuromuscular junction (NMJ), agrin stabilizes, whereas acetylcholine (ACh) destabilizes AChR clusters, leading to the refinement of synaptic connections. The intracellular mechanism underlying this counteractive interaction remains elusive. Here, we show that caspase-3, the effector protease involved in apoptosis, mediates elimination of AChR clusters. We found that caspase-3 was activated by cholinergic stimulation of cultured muscle cells without inducing cell apoptosis and that this activation was prevented by agrin. Interestingly, inhibition of caspase-3 attenuated ACh agonist-induced dispersion of AChR clusters. Furthermore, we identified Dishevelled1 (Dvl1), a Wnt signaling protein involved in AChR clustering, as the substrate of caspase-3. Blocking Dvl1 cleavage prevented induced dispersion of AChR clusters. Finally, inhibition or genetic ablation of caspase-3 or expression of a caspase-3-resistant form of Dvl1 caused stabilization of aneural AChR clusters. Thus, caspase-3 plays an important role in the elimination of postsynaptic structures during the development of NMJs. |
| 学科主题 | Cell Biology ; Developmental Biology |
| 资助信息 | National Natural Science Foundation of China [31330032, 31321091, 61327902]; Key State Research Program [2014CB910203] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2014-07-30 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2615]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Wang, JY,Chen, F,Fu, XQ,et al. Caspase-3 Cleavage of Dishevelled Induces Elimination of Postsynaptic Structures[J]. DEVELOPMENTAL CELL,2014,28(6):670-684. |
| APA | Wang, JY.,Chen, F.,Fu, XQ.,Ding, CS.,Zhou, L.,...&Luo, ZG.(2014).Caspase-3 Cleavage of Dishevelled Induces Elimination of Postsynaptic Structures.DEVELOPMENTAL CELL,28(6),670-684. |
| MLA | Wang, JY,et al."Caspase-3 Cleavage of Dishevelled Induces Elimination of Postsynaptic Structures".DEVELOPMENTAL CELL 28.6(2014):670-684. |
入库方式： OAI收割
来源：上海神经科学研究所
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