Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung's Disease
文献类型：期刊论文
| 作者 | Chen, ZH ; Zhang, YC ; Jiang, WF ; Yang, C ; Zou, GM ; Kong, Y ; Cai, W |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:1页码:1710-1724 |
| 关键词 | GASTROINTESTINAL STROMAL TUMORS ENTERIC NERVOUS-SYSTEM MOUSE SMALL-INTESTINE STEM-CELLS C-KIT ELECTRICAL RHYTHMICITY CHINESE POPULATION HUMAN STOMACH COLON ICC |
| ISSN号 | 1932-6203 |
| 通讯作者 | Cai, W (reprint author), Shanghai Jiao Tong Univ, Sch Med, Xin Hua Hosp, Shanghai Inst Pediat Res, Shanghai 200030, Peoples R China.,Caiw1978@163.com |
| 英文摘要 | Interstitial cells of Cajal (ICC) are critical to gastrointestinal motility. The phenotypes of ICC progenitors have been observed in the mouse gut, but whether they exist in the human colon and what abnormal changes in their quantity and ultrastructure are present in Hirschsprung's disease (HSCR) colon remains uncertain. In this study, we collected the surgical resection of colons, both proximal and narrow segments, from HSCR patients and normal controls. First, we identified the progenitor of ICC in normal adult colon using immunofluorescent localization techniques with laser confocal microscopy. Next, the progenitors were sorted to observe their morphology. We further applied flow cytometry to examine the content of ICC progenitors in these fresh samples. The ultrastructural changes in the narrow and proximal parts of the HSCR colon were observed using transmission electron microscopy (TEM) and were compared with the normal adult colon. The presumed early progenitor (c-Kit(low)CD34(+)Igf1r(+)) and committed progenitor (c-Kit(+)CD34(+)Igf1r(+)) of ICC exist in adult normal colon as well as in the narrow and proximal parts of the HSCR colon. However, the proportions of mature, early and committed progenitors of ICC were dramatically reduced in the narrow segment of the HSCR colon. The proportions of mature and committed progenitors of ICC in the proximal segment of the HSCR colon were lower than in the adult normal colon. Ultrastructurally, ICC, enteric nerves, and smooth muscle in the narrow segment of the HSCR colon showed severe injury, including swollen vacuola or ted mitochondria, disappearance of mitochondrial cristae, dilated rough endoplasmic reticulum, vesiculation and degranulation, and disappearance of the caveolae on the ICC membrane surface. The contents of ICC and its progenitors in the narrow part of the HSCR colon were significantly decreased than those of adult colon, which may be associated with HSCR pathogenesis. |
| 学科主题 | Multidisciplinary Sciences |
| 收录类别 | SCI |
| 资助信息 | Shanghai Jiao Tong University Innovation Team Fund |
| 语种 | 英语 |
| 公开日期 | 2014-07-30 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2627]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Chen, ZH,Zhang, YC,Jiang, WF,et al. Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung's Disease[J]. PLOS ONE,2014,9(1):1710-1724. |
| APA | Chen, ZH.,Zhang, YC.,Jiang, WF.,Yang, C.,Zou, GM.,...&Cai, W.(2014).Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung's Disease.PLOS ONE,9(1),1710-1724. |
| MLA | Chen, ZH,et al."Characterization of Interstitial Cajal Progenitors Cells and Their Changes in Hirschsprung's Disease".PLOS ONE 9.1(2014):1710-1724. |
入库方式： OAI收割
来源：上海神经科学研究所
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