Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay
文献类型：期刊论文
| 作者 | Guo, Yongyong; Zhou, Bingsheng |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2013-10-15 |
| 卷号 | 142期号:-页码:138-145 |
| 关键词 | Pentachlorophenol T-Screen assay Zebrafish larvae Thyroid endocrine disruption Hypothalamic-pituitary-thyroid (HPT) axis |
| ISSN号 | 0166-445X |
| 通讯作者 | Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | The present study aimed to evaluate the disruption caused to the thyroid endocrine system by pentachlorophenol (PCP) using in vitro and in vivo assays. In the in vitro assay, rat pituitary GH3 cells were exposed to 0, 0.1, 0.3, and 1.0 mu M PCP. PCP exposure significantly downregulated basal and tri-iodothyronine (T3)-induced Dio 1 transcription, indicating the antagonistic activity of PCP in vitro. In the in vivo assay, zebrafish embryos were exposed to 0, 1, 3, and 10 mu g/L of PCP until 14 days post-fertilization. PCP exposure resulted in decreased thyroxine (T4) levels, but elevated contents of whole-body T3. PCP exposure significantly upregulated the mRNA expression of genes along hypothalamic-pituitary-thyroid (HPT) axis, including those encoding thyroid-stimulating hormone, sodium/iodide symporter, thyroglobulin, Dio 1 and Dio 2, alpha and beta thyroid hormone receptor, and uridinediphosphate-glucuronosyl-transferase. PCP exposure did not influence the transcription of the transthyretin (TTR) gene. The results indicate that PCP potentially disrupts the thyroid endocrine system both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; HORMONE RECEPTOR ALPHA-1 ; ZEBRAFISH DANIO-RERIO ; PITUITARY-TUMOR CELLS ; RAT GH3 CELLS ; GROWTH-HORMONE ; GENE-EXPRESSION ; XENOPUS-LAEVIS ; T-SCREEN |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [21237005]; Chinese Academy of Sciences [KZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] |
| 语种 | 英语 |
| WOS记录号 | WOS:000328093900014 |
| 公开日期 | 2014-08-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19981]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yongyong,Zhou, Bingsheng. Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay[J]. AQUATIC TOXICOLOGY,2013,142(-):138-145. |
| APA | Guo, Yongyong,&Zhou, Bingsheng.(2013).Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay.AQUATIC TOXICOLOGY,142(-),138-145. |
| MLA | Guo, Yongyong,et al."Thyroid endocrine system disruption by pentachlorophenol: An in vitro and in vivo assay".AQUATIC TOXICOLOGY 142.-(2013):138-145. |
入库方式： OAI收割
来源：水生生物研究所
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