Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate
文献类型：期刊论文
| 作者 | Han, Zhihua1,2; Wang, Qiangwei3; Fu, Jie1,2; Chen, Hongshan4,5; Zhao, Ye5; Zhou, Bingsheng3; Gong, Zhiyuan5; Wei, Si1,2; Li, Jun1,2; Liu, Hongling1,2 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 150期号:-页码:175-181 |
| 关键词 | Developmental toxicity Zebrafish iTRAQ Flame retardants TBEP |
| ISSN号 | 0166-445X |
| 通讯作者 | Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China. |
| 中文摘要 | The flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | The flame retardant tris(2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment, wildlife and human milk. The potentially toxic effects of TBEP and their underlying molecular mechanisms have not been elucidated. Here, zebrafish embryos were exposed to different concentrations of TBEP from 4 hours of post-fertilization (hpf) to 120 hpf, and effects on embryonic development and global protein expression patterns examined. Our results demonstrate that treatment with TBEP (0.8-100 mg/L) causes a concentration- and time-dependent decrease in embryonic survival and the hatching percentage. The median lethal concentration was 10.7 mg/L at 120 hpf. Furthermore, exposure to 150 or 800 mu g/L TBEP inhibited the degradation and utilization of vitellogenins and down-regulated the expression of proteins related to cation binding, and lipid transport, uptake and metabolism, accompanied by a decrease in heart rate and body length. Exposure to TBEP (150 or 800 mu g/L) also decreased the expression of proteins involved in cell proliferation and DNA repair, and led to an increased number of apoptotic cells in the tail region. Collectively, our results suggest that exposure to TBEP causes toxicity in the developing zebrafish by inhibiting the degradation and utilization of nutrients from the mother and inducing apoptosis. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | ORGANOPHOSPHORUS FLAME RETARDANTS ; MESSENGER-RNA EXPRESSION ; TRIS(1,3-DICHLORO-2-PROPYL) PHOSPHATE ; MASS-SPECTROMETRY ; XENOPUS-LAEVIS ; PLASTICIZERS ; PROTEINS ; FISH ; CHROMATOGRAPHY ; TRANSCRIPTION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [21207063, 21377053]; Environmental Protection Department of Jiangsu Province [2012044] |
| 语种 | 英语 |
| WOS记录号 | WOS:000336187900020 |
| 公开日期 | 2014-08-08 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20065]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China 2.Nanjing Univ, Sch Environm, Nanjing 210023, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.NE Normal Univ, Sch Environm, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130024, Peoples R China 5.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore 6.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Zhihua,Wang, Qiangwei,Fu, Jie,et al. Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate[J]. AQUATIC TOXICOLOGY,2014,150(-):175-181. |
| APA | Han, Zhihua.,Wang, Qiangwei.,Fu, Jie.,Chen, Hongshan.,Zhao, Ye.,...&Yu, Hongxia.(2014).Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate.AQUATIC TOXICOLOGY,150(-),175-181. |
| MLA | Han, Zhihua,et al."Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate".AQUATIC TOXICOLOGY 150.-(2014):175-181. |
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来源：水生生物研究所
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