Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library
文献类型：期刊论文
| 作者 | Liu, Hong1,3; Zheng, Xiaocong1; Zhang, Feng2; Yu, Li1; Zhang, Xiaohua2; Dai, Heping2; Hua, Qunyi1; Shi, Xiujie1; Lan, Wensheng1; Jia, Peng1 |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 194期号:1-2页码:178-184 |
| 关键词 | Phage display Single-chain fragment variable Spring viraemia of carp virus |
| ISSN号 | 0166-0934 |
| 通讯作者 | Yuan, L (reprint author), Chinese Acad Sci, Inst Hydrobiol, East Lake Southern Rd 7, Wuhan 430072, Hubei, Peoples R China. |
| 中文摘要 | Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | MEDIATED ISOTHERMAL AMPLIFICATION ; CYPRINUS-CARPIO ; COMMON CARP ; RHABDOVIRUS ; TECHNOLOGY ; SEQUENCE ; CELLS ; SVCV |
| 收录类别 | SCI |
| 资助信息 | China's General Administration for Quality Supervision, Inspection and Quarantine [201210055, 201210214] |
| 语种 | 英语 |
| WOS记录号 | WOS:000328521600028 |
| 公开日期 | 2014-08-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19983]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hong,Zheng, Xiaocong,Zhang, Feng,et al. Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library[J]. JOURNAL OF VIROLOGICAL METHODS,2013,194(1-2):178-184. |
| APA | Liu, Hong.,Zheng, Xiaocong.,Zhang, Feng.,Yu, Li.,Zhang, Xiaohua.,...&Liu, Hong.(2013).Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library.JOURNAL OF VIROLOGICAL METHODS,194(1-2),178-184. |
| MLA | Liu, Hong,et al."Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library".JOURNAL OF VIROLOGICAL METHODS 194.1-2(2013):178-184. |
入库方式： OAI收割
来源：水生生物研究所
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