Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins
文献类型：期刊论文
| 作者 | Zhao, Yanyan1; Xie, Ping1,2; Fan, Huihui2; Zhao, Sujuan1 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 29期号:1页码:30-39 |
| 关键词 | microcystins mitochondrion impairment OXPHOS system crucian carp |
| ISSN号 | 1520-4081 |
| 通讯作者 | Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China. |
| 中文摘要 | Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014. |
| 英文摘要 | Hepatotoxic microcystins (MCs) are produced by cyanobacteria in diverse water bodies and the pathophysiology includes induction of reactive oxygen species and adenosine triphosphate (ATP) depletion in cells. In this study, we evaluated MCs induced changes in the oxidative phosphorylation (OXPHOS) system in mitochondria of crucian carp liver. Fish were subdivided into two groups that were intraperitoneally injected with two doses of MCs (50 and 200 MC-LReq g/kg bw) and were sacrificed at 1, 3, 12, 24, and 48 h postinjection. The activities of five enzyme complexes of electron transport chain and mRNA expression of mitochondrial-encoded genes (cox1, cox2, cox3, and atp6) were significantly reduced in a time-dependent pattern after injection. There were also changes in mitochondrial ultrastructure, decreases in ATPase activities and reduction in antioxidant level after MCs exposure. Disorder in the OXPHOS system and decreased activities of antioxidative enzymes might contribute to bioenergy deficiency and consequent hepatocyte damage induced by MCs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol 29: 30-39, 2014. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | ELECTRON-TRANSPORT CHAIN ; EXTRACTED MICROCYSTINS ; INDUCED APOPTOSIS ; CELL-DEATH ; IN-VIVO ; DAMAGE ; LR ; GENERATION ; MUSCLE ; HEART |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2008CB418101]; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000328545400004 |
| 公开日期 | 2014-08-07 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20031]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yanyan,Xie, Ping,Fan, Huihui,et al. Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins[J]. ENVIRONMENTAL TOXICOLOGY,2014,29(1):30-39. |
| APA | Zhao, Yanyan,Xie, Ping,Fan, Huihui,&Zhao, Sujuan.(2014).Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins.ENVIRONMENTAL TOXICOLOGY,29(1),30-39. |
| MLA | Zhao, Yanyan,et al."Impairment of the Mitochondrial Oxidative Phosphorylation System and Oxidative Stress in Liver of Crucian Carp (Carassius auratus L.) Exposed to Microcystins".ENVIRONMENTAL TOXICOLOGY 29.1(2014):30-39. |
入库方式： OAI收割
来源：水生生物研究所
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