中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)

文献类型:期刊论文

作者Chen, Y.1,2; Zhu, X.1; Yang, Y.1; Han, D.1; Jin, J.1; Xie, S.1
刊名AQUACULTURE NUTRITION
出版日期2014-06-01
卷号20期号:3页码:229-241
关键词Aeromonas hydrophilia Carassius auratus gibelio immunity intestine microbiota intestine morphology lysozyme
ISSN号1353-5773
通讯作者Xie, S (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.
英文摘要A 75days experiment was conducted in a flow-through system on juvenile gibel carp (Carassius auratus gibelio) (3.43 +/- 0.01g) to evaluate the effects of dietary lysozyme on growth performance, intestine morphology, microbiota and immune response. Four isonitrogenous (crude protein: 367 g kg(-1)) isolipid (62 g kg(-1)) and isocaloric (gross energy: 17.92kJg(-1)) diets were formulated to contain 0, 100, 500 and 1000mgkg(-1) lysozyme, respectively. The results showed that specific growth rate (SGR) and feed efficiency (FE) increased at 1000mgkg(-1) lysozyme. Blood leucocyte phagocytic activity (PA) and serum lysozyme (LZM) decreased with dietary lysozyme on day 25, 50 and 75. There were no significant differences in alternative complement pathway (ACP), respiratory burst (ROS), serum superoxide dismutase (SOD), glutathione peroxidase (GSHpx) or malonaldehyde (MDA). After Aeromonas hydrophilia challenge, higher survival was obtained at 500mgkg(-1) group. PA, ROS, SOD, LZM and ACP increased with increasing dietary lysozyme, while MDA reversed. Goblet cells in mid-intestine and microvilli height in distal intestine increased with dietary lysozyme on day 75. Dietary lysozyme reduced the diversity of intestine microbiota. In conclusion, oral administration of 500mgkg(-1) dietary lysozyme for 75days is recommended for the survival of gibel carp and 1000mgkg(-1) dietary lysozyme for fast growth.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Fisheries
研究领域[WOS]Fisheries
关键词[WOS]POLYMORPHONUCLEAR LEUKOCYTES ; EPITHELIAL-CELLS ; CHANNEL CATFISH ; HYBRID TILAPIA ; GERM-FREE ; PROBIOTICS ; FISH ; INFLAMMATION ; ANTIBIOTICS ; ANTIGEN
收录类别SCI
资助信息National Key Basic Research Program (NKBRP) [2009CB118702]; earmarked fund for China Agriculture Research System [CARS-46-19]; Special Fund for Agro-scientific Research in the Public Interest [201003020]
语种英语
WOS记录号WOS:000336258300001
公开日期2014-08-07
源URL[http://ir.ihb.ac.cn/handle/342005/20032]  
专题水生生物研究所_淡水生态学研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Chen, Y.,Zhu, X.,Yang, Y.,et al. Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)[J]. AQUACULTURE NUTRITION,2014,20(3):229-241.
APA Chen, Y.,Zhu, X.,Yang, Y.,Han, D.,Jin, J.,&Xie, S..(2014).Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio).AQUACULTURE NUTRITION,20(3),229-241.
MLA Chen, Y.,et al."Effect of dietary lysozyme on growth, immune response intestine microbiota, intestine morphology and resistance to Aeromonas hydrophilia in gibel carp (Carassius auratus gibelio)".AQUACULTURE NUTRITION 20.3(2014):229-241.

入库方式: OAI收割

来源:水生生物研究所

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