Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish
文献类型：期刊论文
| 作者 | Dan, Cheng1; Mei, Jie2; Wang, Da1; Gui, Jian-Fang1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
 |
| 出版日期 | 2013 |
| 卷号 | 9期号:10页码:1043-1049 |
| 关键词 | Yellow catfish Sex-specific marker genome walking YY super-males All-male fish |
| ISSN号 | 1449-2288 |
| 通讯作者 | Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China. |
| 中文摘要 | Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish. |
| 英文摘要 | Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | SOLE CYNOGLOSSUS-SEMILAEVIS ; PELTEOBAGRUS-FULVIDRACO RICHARDSON ; CARASSIUS-AURATUS GIBELIO ; AFLP-BASED APPROACH ; CYPRINUS-CARPIO ; CLONAL DIVERSITY ; DNA FRAGMENTS ; COMMON CARP ; LINKAGE MAP ; IDENTIFICATION |
| 收录类别 | SCI |
| 资助信息 | special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [2009030406]; National Key Basic Research Program [2010CB126301]; Innovation Project of Chinese Academy of Sciences [KSCX2-EW-N-04]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] |
| 语种 | 英语 |
| WOS记录号 | WOS:000330121700004 |
| 公开日期 | 2014-08-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19972]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Minist Agr, Coll Fisheries,Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dan, Cheng,Mei, Jie,Wang, Da,et al. Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(10):1043-1049. |
| APA | Dan, Cheng,Mei, Jie,Wang, Da,&Gui, Jian-Fang.(2013).Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,9(10),1043-1049. |
| MLA | Dan, Cheng,et al."Genetic Differentiation and Efficient Sex-specific Marker Development of a Pair of Y- and X-linked Markers in Yellow Catfish".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.10(2013):1043-1049. |
入库方式： OAI收割
来源：水生生物研究所
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