Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families
文献类型：期刊论文
| 作者 | Zhu, Chuankun1,2; Sun, Yanhong1,2; Yu, Xiaomu1; Tong, Jingou1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-12-20 |
| 卷号 | 8期号:12页码:- |
| 关键词 | OYSTER CRASSOSTREA-GIGAS MICROSATELLITE LINKAGE MAP TROUT ONCORHYNCHUS-MYKISS RAINBOW-TROUT RECOMBINATION RATES TRIPLOID FAMILIES SEA-CUCUMBER GENETIC-MAP LOCI ZEBRAFISH |
| ISSN号 | 1932-6203 |
| 通讯作者 | Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China. |
| 中文摘要 | Gene-centromere (G-C) mapping provides insights into structural and behavioural properties of chromosomes. In this study, G-C mapping using microsatellite markers and meiogynogenetic (meiotic gynogenetic) families were performed in bighead carp (Aristichthys nobilis, 2N = 48), which belongs to Cyprinidae. A total of 218 microsatellites were selected across 24 linkage groups (LGs) of a recently well-defined genetic linkage map for bighead carp, with 151 being heterozygous in at least one of six dams in diploid meiogynogenetic families. After tests for Mendelian segregation in two diploid control families, 103 microsatellites were used for G-C distance calculation in 383 gynogens. The second division segregation frequency (y) was computed through half-tetrad analyses, and the values ranged from 0 to 0.97 (mean 0.40). High G-C recombination frequencies (over 0.667) were observed in 18 (17.5%) of the loci examined, which revealed a low level of chiasma interferences compared with other fishes studied previously. Distribution of G-C distances across LGs ranged from 0 cM to 48.5 cM (mean 20 cM) under the assumption of complete interference. All 24 centromeres were localized according to their closest-related microsatellites at 95% confident intervals. The average distance between centromeres and their closest-linked markers was 6.1 cM with 15 out of 24 LGs having a distance below 5 cM. Based on the centromere positions in this study, we proposed a formula of 24 m/sm+24 t/st chromosomes with 92 arms for bighead carp, which was mostly in accordance with a previously reported karyotype for bighead carp (24 m/sm+24 st). These results of centromere localization provide a basic framework and important resources for genetics and comparative genomics studies in bighead carp and its closely-related cyprinid species. |
| 英文摘要 | Gene-centromere (G-C) mapping provides insights into structural and behavioural properties of chromosomes. In this study, G-C mapping using microsatellite markers and meiogynogenetic (meiotic gynogenetic) families were performed in bighead carp (Aristichthys nobilis, 2N = 48), which belongs to Cyprinidae. A total of 218 microsatellites were selected across 24 linkage groups (LGs) of a recently well-defined genetic linkage map for bighead carp, with 151 being heterozygous in at least one of six dams in diploid meiogynogenetic families. After tests for Mendelian segregation in two diploid control families, 103 microsatellites were used for G-C distance calculation in 383 gynogens. The second division segregation frequency (y) was computed through half-tetrad analyses, and the values ranged from 0 to 0.97 (mean 0.40). High G-C recombination frequencies (over 0.667) were observed in 18 (17.5%) of the loci examined, which revealed a low level of chiasma interferences compared with other fishes studied previously. Distribution of G-C distances across LGs ranged from 0 cM to 48.5 cM (mean 20 cM) under the assumption of complete interference. All 24 centromeres were localized according to their closest-related microsatellites at 95% confident intervals. The average distance between centromeres and their closest-linked markers was 6.1 cM with 15 out of 24 LGs having a distance below 5 cM. Based on the centromere positions in this study, we proposed a formula of 24 m/sm+24 t/st chromosomes with 92 arms for bighead carp, which was mostly in accordance with a previously reported karyotype for bighead carp (24 m/sm+24 st). These results of centromere localization provide a basic framework and important resources for genetics and comparative genomics studies in bighead carp and its closely-related cyprinid species. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; MICROSATELLITE LINKAGE MAP ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; RECOMBINATION RATES ; TRIPLOID FAMILIES ; SEA-CUCUMBER ; GENETIC-MAP ; LOCI ; ZEBRAFISH |
| 收录类别 | SCI |
| 资助信息 | NSFC of China [31272647]; MOA of China [200903045, 2011-G12]; FEBL of China [2011FBZ20] |
| 语种 | 英语 |
| WOS记录号 | WOS:000328745100074 |
| 公开日期 | 2014-08-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19975]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Chuankun,Sun, Yanhong,Yu, Xiaomu,et al. Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families[J]. PLOS ONE,2013,8(12):-. |
| APA | Zhu, Chuankun,Sun, Yanhong,Yu, Xiaomu,&Tong, Jingou.(2013).Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families.PLOS ONE,8(12),-. |
| MLA | Zhu, Chuankun,et al."Centromere Localization for Bighead Carp (Aristichthys nobilis) through Half-Tetrad Analysis in Diploid Gynogenetic Families".PLOS ONE 8.12(2013):-. |
入库方式： OAI收割
来源：水生生物研究所
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