Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp
文献类型：期刊论文
| 作者 | Zhai, Yan-Hua1; Zhou, Li1; Wang, Yang1; Wang, Zhong-Wei1; Li, Zhi1; Zhang, Xiao-Juan1; Gui, Jian-Fang1,2 |
| 刊名 | PARASITOLOGY RESEARCH
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 113期号:4页码:1331-1341 |
| 关键词 | CARASSIUS-AURATUS-GIBELIO APOLIPOPROTEIN-A-I ANTIMICROBIAL PEPTIDES ANTIINFECTIVE ACTIVITY PRIMARY DEFENSE MOLECULAR-DATA MYXOZOA BLOCH CELL IDENTIFICATION |
| ISSN号 | 0932-0113 |
| 通讯作者 | Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China. |
| 中文摘要 | Some myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease. |
| 英文摘要 | Some myxosporeans have been demonstrated to be harmful to worldwide aquaculture. However, the proliferation information has remained unclear in the fish hosts. In this study, we utilized the mix-culturing equality to reveal significant difference in disease assistance between two different clones of gibel carp, in which clone D had been cultured for nearly 40 years, whereas clone A(+) was a newly created clone. According to morphological and genetic analysis of isolated spores, the diseasing pathogen was identified as Myxobolus wulii of the genus Myxobolus in Myxosporea. Subsequently, a polyclonal antibody specific to soluble proteins of the purified spores was generated. Using the antibody, we performed immunofluorescence observation of the liver lump sections sampled from the heavily diseased clone D individuals, and found that the liver lumps were completely composed of numerous honeycomb-like cysts, full of maturing and mature myxosporean spores, and almost all of liver tissues were destroyed. Comparative co-localization detection revealed a significantly inducing expression of apo-14 protein around the infected myxosporean sporoplasms and plasmodia, and the inducing level was much stronger in clone A(+) than in clone D. Furthermore, a primarily screening of 15 different major histocompatibility complex class I alpha variants also excavated major variants that respectively belong to clones D and A(+). Therefore, these data provide significant information for differences in myxosporean proliferation and disease resistance in fish clone hosts with different genetic background. Further studies on myxosporean development and the mechanism for disease resistance will be very important for preventing and controlling the parasitic myxosporean disease. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Parasitology |
| 研究领域[WOS] | Parasitology |
| 关键词[WOS] | CARASSIUS-AURATUS-GIBELIO ; APOLIPOPROTEIN-A-I ; MYXOZOA MYXOSPOREA ; ANTIMICROBIAL PEPTIDES ; ANTIINFECTIVE ACTIVITY ; PRIMARY DEFENSE ; MOLECULAR-DATA ; BLOCH ; CELL ; IDENTIFICATION |
| 收录类别 | SCI |
| 资助信息 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA08030202]; special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China [200903045]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; National Science and Technology Support Program [2012BA026B02]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ22] |
| 语种 | 英语 |
| WOS记录号 | WOS:000333029800010 |
| 公开日期 | 2014-08-08 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20044]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Yan-Hua,Zhou, Li,Wang, Yang,et al. Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp[J]. PARASITOLOGY RESEARCH,2014,113(4):1331-1341. |
| APA | Zhai, Yan-Hua.,Zhou, Li.,Wang, Yang.,Wang, Zhong-Wei.,Li, Zhi.,...&Gui, Jian-Fang.(2014).Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp.PARASITOLOGY RESEARCH,113(4),1331-1341. |
| MLA | Zhai, Yan-Hua,et al."Proliferation and resistance difference of a liver-parasitized myxosporean in two different gynogenetic clones of gibel carp".PARASITOLOGY RESEARCH 113.4(2014):1331-1341. |
入库方式： OAI收割
来源：水生生物研究所
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