IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis
文献类型：期刊论文
| 作者 | Fu, Jian Ping1,2; Chen, Shan Nan1; Zou, Peng Fei1; Huang, Bei3; Guo, Zheng1; Zeng, Ling Bing4; Qin, Qi Wei5; Nie, Pin1,3 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 43期号:1页码:87-95 |
| 关键词 | IFN-gamma IFN-gamma stimulated gene Iridovirus Antiviral activity Turtle |
| ISSN号 | 0145-305X |
| 通讯作者 | Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. |
| 中文摘要 | The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | NUCLEAR-LOCALIZATION SEQUENCE ; TROUT ONCORHYNCHUS-MYKISS ; DOUBLE-STRANDED-RNA ; INTERFERON-GAMMA ; ANTIVIRAL ACTIVITY ; TELEOST FISH ; BODY PLAN ; II IFNS ; EXPRESSION ; EVOLUTION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] |
| 语种 | 英语 |
| WOS记录号 | WOS:000331479800012 |
| 公开日期 | 2014-08-08 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20048]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China 4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei Province, Peoples R China 5.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Jian Ping,Chen, Shan Nan,Zou, Peng Fei,et al. IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):87-95. |
| APA | Fu, Jian Ping.,Chen, Shan Nan.,Zou, Peng Fei.,Huang, Bei.,Guo, Zheng.,...&Nie, Pin.(2014).IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),87-95. |
| MLA | Fu, Jian Ping,et al."IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):87-95. |
入库方式： OAI收割
来源：水生生物研究所
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