Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants
文献类型：期刊论文
| 作者 | Zhu, Rong; Chen, Zhong-yuan; Wang, Jun; Yuan, Jiang-di; Liao, Xiang-yong; Gui, Jian-Fang; Zhang, Qi-Ya |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 42期号:2页码:311-322 |
| 关键词 | MHC of Chinese giant salamanders Andrias davidianus (Anda-MHC) Genetic diversity Alternative splice variant Andrias davidianus ranavirus (ADRV) Inducible expression |
| ISSN号 | 0145-305X |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding region (PBR), which is driven by positive Darwinian selection. The coexistence of two isoforms in MHC IA, IIA, and IIB alleles are shown: one full-length transcript and one novel splice variant. Despite lake of the external domains, these variants exhibit similar subcellular localization with the full-length transcripts. Moreover, the expression of MHC isoforms are up-regulated upon in vivo and in vitro stimulation with Andrias davidianus ranavirus (ADRV), suggesting their potential roles in the immune response. The results provide insights into understanding MHC variation and function in this ancient and endangered urodele amphibian. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | MAJOR HISTOCOMPATIBILITY COMPLEX ; WILD TIGER SALAMANDERS ; CLASS-I GENES ; MOLECULAR CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SELECTION ; POLYMORPHISM ; MECHANISMS ; EVOLUTION ; RANAVIRUS |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31372551, 31202028, 31072231, 31072239] |
| 语种 | 英语 |
| WOS记录号 | WOS:000328725800021 |
| 公开日期 | 2014-08-08 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20059]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Rong,Chen, Zhong-yuan,Wang, Jun,et al. Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,42(2):311-322. |
| APA | Zhu, Rong.,Chen, Zhong-yuan.,Wang, Jun.,Yuan, Jiang-di.,Liao, Xiang-yong.,...&Zhang, Qi-Ya.(2014).Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,42(2),311-322. |
| MLA | Zhu, Rong,et al."Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 42.2(2014):311-322. |
入库方式： OAI收割
来源：水生生物研究所
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