AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis
文献类型：期刊论文
| 作者 | Yan, An1; Wu, Minjie1; Yan, Limei1; Hu, Rui2; Ali, Imran1; Gan, Yinbo1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-01-03 |
| 卷号 | 9期号:1页码:e85208 |
| 关键词 | CELL-WALL EXTENSIBILITY EXPANSIN GENE GIBBERELLIN BIOSYNTHESIS MOLECULAR-CLONING MULTIGENE FAMILY LEAF DEVELOPMENT EXPRESSION THALIANA PROTEINS GROWTH |
| ISSN号 | 1932-6203 |
| 通讯作者 | Gan, YB (reprint author), Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China. |
| 中文摘要 | Expansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis. |
| 英文摘要 | Expansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CELL-WALL EXTENSIBILITY ; EXPANSIN GENE ; GIBBERELLIN BIOSYNTHESIS ; MOLECULAR-CLONING ; MULTIGENE FAMILY ; LEAF DEVELOPMENT ; EXPRESSION ; THALIANA ; PROTEINS ; GROWTH |
| 收录类别 | SCI |
| 资助信息 | International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] |
| 语种 | 英语 |
| WOS记录号 | WOS:000329460800082 |
| 公开日期 | 2014-08-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20125]  |
| 专题 | 水生生物研究所_分析测试中心_期刊论文 |
| 作者单位 | 1.Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, An,Wu, Minjie,Yan, Limei,et al. AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis[J]. PLOS ONE,2014,9(1):e85208. |
| APA | Yan, An,Wu, Minjie,Yan, Limei,Hu, Rui,Ali, Imran,&Gan, Yinbo.(2014).AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis.PLOS ONE,9(1),e85208. |
| MLA | Yan, An,et al."AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis".PLOS ONE 9.1(2014):e85208. |
入库方式： OAI收割
来源：水生生物研究所
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