Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis
文献类型：期刊论文
| 作者 | Ye, Jun1; Chang, Yue2; Yan, Yu1,3; Xiong, Jie2; Xue, Xi-Mei1,3; Yuan, Dongxia2; Sun, Guo-Xin4; Zhu, Yong-Guan1,4; Miao, Wei2 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 149页码:50-57 |
| ISSN号 | 0166-445X |
| 关键词 | Tetrahymena pyriformis Protozoan Arsenic Methyltransferase Methylarsine Heavy metal |
| 通讯作者 | Miao, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. |
| 中文摘要 | Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coil; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | S-ADENOSYLMETHIONINE METHYLTRANSFERASE ; MARINE FOOD-CHAIN ; TETRAHYMENA-THERMOPHILA ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; SEQUENCE ; BIOTRANSFORMATION ; BIOMETHYLATION ; TRANSFERASE ; METHYLATION |
| 资助信息 | National Science Foundation of China [31372168, 31270161]; HUbei [ZRZ0251] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334989300007 |
| 公开日期 | 2014-08-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20087]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Jun,Chang, Yue,Yan, Yu,et al. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis[J]. AQUATIC TOXICOLOGY,2014,149:50-57. |
| APA | Ye, Jun.,Chang, Yue.,Yan, Yu.,Xiong, Jie.,Xue, Xi-Mei.,...&Miao, Wei.(2014).Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis.AQUATIC TOXICOLOGY,149,50-57. |
| MLA | Ye, Jun,et al."Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis".AQUATIC TOXICOLOGY 149(2014):50-57. |
入库方式： OAI收割
来源：水生生物研究所
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