Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development
文献类型：期刊论文
| 作者 | Zhai, Gang1,2; Gu, Qilin1,2; He, Jiangyan1; Lou, Qiyong1; Chen, Xiaowen1; Jin, Xia1; Bi, Erfei3; Yin, Zhan1 |
| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 34期号:7页码:1310-1321 |
| 关键词 | LEFT-RIGHT ASYMMETRY POLYCYSTIC KIDNEY-DISEASE PRIMARY CILIUM SACCHAROMYCES-CEREVISIAE MAMMALIAN SEPTINS BUDDING YEAST INTRAFLAGELLAR TRANSPORT FILAMENT FORMATION DIFFUSION BARRIER GENE SOUTHPAW |
| ISSN号 | 0270-7306 |
| 通讯作者 | Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China. |
| 中文摘要 | Septins are conserved filament-forming GTP-binding proteins that act as cellular scaffolds or diffusion barriers in a number of cellular processes. However, the role of septins in vertebrate development remains relatively obscure. Here, we show that zebrafish septin 6 (sept6) is first expressed in the notochord and then in nearly all of the ciliary organs, including Kupffer's vesicle (KV), the pronephros, eye, olfactory bulb, and neural tube. Knockdown of sept6 in zebrafish embryos results in reduced numbers and length of cilia in KV. Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised. Knockdown of sept6 also results in aberrant cilium formation in the pronephros and neural tube, leading to cilium-related defects in pronephros development and Sonic hedgehog (Shh) signaling. We further demonstrate that SEPT6 associates with acetylated alpha-tubulin in vivo and localizes along the axoneme in the cilia of zebrafish pro-nephric duct cells as well as cultured ZF4 cells. Our study reveals a novel role of sept6 in ciliogenesis during early embryonic development in zebrafish. |
| 英文摘要 | Septins are conserved filament-forming GTP-binding proteins that act as cellular scaffolds or diffusion barriers in a number of cellular processes. However, the role of septins in vertebrate development remains relatively obscure. Here, we show that zebrafish septin 6 (sept6) is first expressed in the notochord and then in nearly all of the ciliary organs, including Kupffer's vesicle (KV), the pronephros, eye, olfactory bulb, and neural tube. Knockdown of sept6 in zebrafish embryos results in reduced numbers and length of cilia in KV. Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised. Knockdown of sept6 also results in aberrant cilium formation in the pronephros and neural tube, leading to cilium-related defects in pronephros development and Sonic hedgehog (Shh) signaling. We further demonstrate that SEPT6 associates with acetylated alpha-tubulin in vivo and localizes along the axoneme in the cilia of zebrafish pro-nephric duct cells as well as cultured ZF4 cells. Our study reveals a novel role of sept6 in ciliogenesis during early embryonic development in zebrafish. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | LEFT-RIGHT ASYMMETRY ; POLYCYSTIC KIDNEY-DISEASE ; PRIMARY CILIUM ; SACCHAROMYCES-CEREVISIAE ; MAMMALIAN SEPTINS ; BUDDING YEAST ; INTRAFLAGELLAR TRANSPORT ; FILAMENT FORMATION ; DIFFUSION BARRIER ; GENE SOUTHPAW |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]; U.S. National Institutes of Health [GM59216, GM87365] |
| 语种 | 英语 |
| WOS记录号 | WOS:000332696800012 |
| 公开日期 | 2014-08-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20090]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA |
| 推荐引用方式 GB/T 7714 | Zhai, Gang,Gu, Qilin,He, Jiangyan,et al. Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development[J]. MOLECULAR AND CELLULAR BIOLOGY,2014,34(7):1310-1321. |
| APA | Zhai, Gang.,Gu, Qilin.,He, Jiangyan.,Lou, Qiyong.,Chen, Xiaowen.,...&Yin, Zhan.(2014).Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development.MOLECULAR AND CELLULAR BIOLOGY,34(7),1310-1321. |
| MLA | Zhai, Gang,et al."Sept6 Is Required for Ciliogenesis in Kupffer's Vesicle, the Pronephros, and the Neural Tube during Early Embryonic Development".MOLECULAR AND CELLULAR BIOLOGY 34.7(2014):1310-1321. |
入库方式： OAI收割
来源：水生生物研究所
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