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Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803

文献类型：期刊论文

作者Chen, Gao1,2; Qu, Shujie5; Wang, Qiang4; Bian, Fei1,2; Peng, Zhenying1,2; Zhang, Yan1,2; Ge, Haitao6; Yu, Jinhui1,2; Xuan, Ning1,2; Bi, Yuping1,2
刊名BIOTECHNOLOGY FOR BIOFUELS
出版日期2014-03-01
卷号7
关键词Omega-3 polyunsaturated fatty acids Gene expression Fatty acid desaturase Synechocystis sp PCC6803
ISSN号1754-6834
通讯作者Bi, YP (reprint author), Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China.
中文摘要Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete. Results: We constructed a series of homologous vectors for the endogenous and exogenous expression of Delta 6 and Delta 15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9 mg/l of alpha-linolenic acid (ALA) and 4.1 mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20 degrees C and 30 degrees C. Thus, transgenic expression of Delta 6 and Delta 15 fatty acid desaturases enhances the accumulation of specific omega-3 PUFAs in Synechocystis sp. PCC6803. Conclusions: In the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and gamma-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential omega-3 PUFAs.
英文摘要Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]STEARIDONIC ACID ; LONG-CHAIN ; SOYBEAN OIL ; CYANOBACTERIUM SYNECHOCYSTIS-6803 ; CARDIOVASCULAR-DISEASE ; RHEUMATOID-ARTHRITIS ; FISH CONSUMPTION ; LINOLENIC ACID ; OILSEED CROPS ; PLANTS
收录类别SCI
资助信息International Science and Technology Cooperation Program of China [2012DFA30450]; Shandong Province 'Taishan Scholar' Foundation [tshw20091014]; Innovation Fund for the Post-Doctoral Program of Shandong Province [201303029]; National Natural Science Foundation of China [31270102]; Innovation Program of the University Institutes of Jinan, Shandong Province [201004044]; National Science Foundation (MCB) [1120153]
语种英语
WOS记录号WOS:000333014600001
公开日期2014-08-13
源URL[http://ir.ihb.ac.cn/handle/342005/20111]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China
3.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
5.Shandong Acad Agr Sci, Test Base Serv Ctr, Jinan 250100, Peoples R China
6.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Gao,Qu, Shujie,Wang, Qiang,et al. Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803[J]. BIOTECHNOLOGY FOR BIOFUELS,2014,7.
APA Chen, Gao.,Qu, Shujie.,Wang, Qiang.,Bian, Fei.,Peng, Zhenying.,...&He, Qingfang.(2014).Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803.BIOTECHNOLOGY FOR BIOFUELS,7.
MLA Chen, Gao,et al."Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803".BIOTECHNOLOGY FOR BIOFUELS 7(2014).

入库方式： OAI收割

来源：水生生物研究所

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