Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China
文献类型：期刊论文
| 作者 | Liu, Yang1,2; Xu, Yao1; Xiao, Peng1,2; Pan, Qianqian1,2; Yu, Gongliang1; Li, Renhui1 |
| 刊名 | HARMFUL ALGAE
 |
| 出版日期 | 2014 |
| 卷号 | 31页码:76-81 |
| 关键词 | Cyanobacteria Dolichospermum Genotype ITS sequence Lake Taihu |
| ISSN号 | 1568-9883 |
| 通讯作者 | Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Lake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | Lake Taihu has been severely eutrophied during the last few decades and dense cyanobacterial blooms have led to a decrease in phytoplankton diversity. The cyanobacterial blooms in Lake Taihu were mainly composed of unicellular colony-forming Microcystis and filamentous heterocystous Dolichospermum (formerly known as planktonic species of Anabaena). In contrast to that of Microcystis spp., the fundamental knowledge about diversity, abundance and dynamics of Dolichospermum populations in Lake Taihu is lacking. The present study was conducted to understand genotypic distribution, dynamics and succession of Dolichospermum populations in Lake Taihu. By sequencing 688 internal transcribed spacer (ITS) regions between the 16S and 23S rRNA genes of Dolichospermum, we were able to confirm that all the sequences were Dolichospermum rather than Aphanizomenon. 118 different genotypes were identified from the obtained sequences, and two genotypes (W-type and L-type) were found to dominate in the lake, representing 36.6% and 26.2% of the total sequences, respectively. These two dominant genotypes of Dolichospermum displayed the significant seasonal pattern. Stepwise regressions analysis revealed that water temperature was associated with the two dominant genotypes. The combined results implied the possible existence of ecotypes in bloom-forming cyanobacteria, probably triggered by water temperature in the lake. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | REAL-TIME PCR ; PROCHLOROCOCCUS ECOTYPES ; MICROCYSTIS-AERUGINOSA ; SPATIOTEMPORAL CHANGES ; ENVIRONMENTAL-FACTORS ; TOXIC MICROCYSTIS ; FLOS-AQUAE ; BLOOMS ; DIVERSITY ; DYNAMICS |
| 收录类别 | SCI |
| 资助信息 | NSFC-JST Project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] |
| 语种 | 英语 |
| WOS记录号 | WOS:000330497500009 |
| 公开日期 | 2014-08-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20120]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Xu, Yao,Xiao, Peng,et al. Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China[J]. HARMFUL ALGAE,2014,31:76-81. |
| APA | Liu, Yang,Xu, Yao,Xiao, Peng,Pan, Qianqian,Yu, Gongliang,&Li, Renhui.(2014).Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China.HARMFUL ALGAE,31,76-81. |
| MLA | Liu, Yang,et al."Genetic analysis on Dolichospermum (Cyanobacteria; sensu Anabaena) populations based on the culture-independent clone libraries revealed the dominant genotypes existing in Lake Taihu, China".HARMFUL ALGAE 31(2014):76-81. |
入库方式： OAI收割
来源：水生生物研究所
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