Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array
文献类型：期刊论文
| 作者 | Qi C(齐财); Zhang H; Liu L(刘丽) ; Yang YK; Kang TF(康腾飞); Hao WX(郝文欣); Jin G(靳刚); Jiang TJ
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-06-18 |
| 卷号 | 4页码:5341 |
| 通讯作者邮箱 | gajin@imech.ac.cn; taijiao@moon.ibp.ac.cn |
| ISSN号 | 2045-2322 |
| 产权排序 | [Qi, Cai; Zhang, Hong; Jiang, Taijiao] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Liu, Li; Kang, Tengfei; Hao, Wenxin; Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Yang, Yinke] Cent S Univ, Xiang Ya Med Sch, Changsha 410083, Hunan, Peoples R China; [Qi, Cai] Chinese Acad Inspect & Quarantine, Inst Equipment Technol, Beijing 100123, Peoples R China |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, 15 Beisihuan West Rd, Beijing 100190, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | The soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins are small and abundant membrane-bound proteins, whose specific interactions mediate membrane fusion during cell fusion or cellular trafficking. In this study, we report the use of a label-free method, called imaging ellipsometer to analyze the interactions among three SNAREs, namely Sec22p, Ykt6p and Sso2p. The SNAREs were immobilized on the silicon wafer and then analyzed in a pairwise mode with microfluidic array, leading us to discover the interactions between Ykt6p and Sso2p, Sec22p and Sso2p. Moreover, by using the real-time function of the imaging ellipsometer, we were able to obtain their association constants (K-A) of about 10(4) M-1. We argue that the use of imaging ellipsometer coupled with microfluidic device will deepen our understanding of the molecular mechanisms underlying membrane fusion process. |
| 学科主题 | Science & Technology - Other Topics |
| 分类号 | 一类 |
| 收录类别 | SCI |
| 资助信息 | This study was supported by Project 973 (2014CB910500), National Natural Science Foundation of China (31300632), National Science and Technology Major Project of the Ministry of Science and Technology of China (2013ZX10004611-002), The National Basic Research Program (Project 973) of China (2009CB320300), The National Basic Research Program of China (2009CB320302), The National High Technology Research Development Program of China (2008AA02Z419), The International Science & Technology Cooperation Program of China (2012DFG31880), Projects of Sino-Portugal S&T Cooperation 2010-2015, The National Natural Science Foundation of China (21305147), The Instrument Developing Project of the Chinese Academy of Sciences (KJCX2-YW-M04, KJCX2-YW-M03), Basic Scientific Research Special Foundation of Chinese Academy of Inspection and Quarantine (2010JK002) and China Postdoctoral Science Foundation funded project (20080440533). |
| 原文出处 | http://dx.doi.org/10.1038/srep05341 |
| 语种 | 英语 |
| WOS记录号 | WOS:000337499100006 |
| 公开日期 | 2014-09-03 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/49035]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Qi C,Zhang H,Liu L,et al. Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array[J]. SCIENTIFIC REPORTS,2014,4:5341. |
| APA | Qi C.,Zhang H.,Liu L.,Yang YK.,Kang TF.,...&Jiang TJ.(2014).Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array.SCIENTIFIC REPORTS,4,5341. |
| MLA | Qi C,et al."Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array".SCIENTIFIC REPORTS 4(2014):5341. |
入库方式： OAI收割
来源：力学研究所
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