Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis
文献类型：期刊论文
| 作者 | Ma, Qingjun; Akhter, Yusuf; Wilmanns, Matthias; Ehebauer, Matthias T.; Ma, Qingjun; Akhter, Yusuf; Wilmanns, Matthias; Ehebauer, Matthias T. |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2014-07 |
| 卷号 | 23期号:7页码:932-939 |
| 关键词 | Mycobacterium tuberculosis BPL BirA biotinylation crystal structure ACETYL-COA CARBOXYLASE ESCHERICHIA-COLI HOLOENZYME SYNTHETASE CARRIER PROTEIN BIRA GENE SPECIFICITY BIOTINYLATION PURIFICATION ACTIVATION COMPLEXES |
| 中文摘要 | |
| 英文摘要 | Protein biotinylation, a rare form of post-translational modification, is found in enzymes required for lipid biosynthesis. In mycobacteria, this process is essential for the formation of their complex and distinct cell wall and has become a focal point of drug discovery approaches. The enzyme responsible for this process, biotin protein ligase, substantially varies in different species in terms of overall structural organization, regulation of function and substrate specificity. To advance the understanding of the molecular mechanism of biotinylation in Mycobacterium tuberculosis we have biochemically and structurally characterized the corresponding enzyme. We report the high-resolution crystal structures of the apo-form and reaction intermediate biotinyl-5'-AMP-bound form of M. tuberculosis biotin protein ligase. Binding of the reaction intermediate leads to clear disorder-to-order transitions. We show that a conserved lysine, Lys138, in the active site is essential for biotinylation. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2014-09-08 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/18197]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Ma, Qingjun,Akhter, Yusuf,Wilmanns, Matthias,et al. Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis[J]. PROTEIN SCIENCE,2014,23(7):932-939. |
| APA | Ma, Qingjun.,Akhter, Yusuf.,Wilmanns, Matthias.,Ehebauer, Matthias T..,Ma, Qingjun.,...&Ehebauer, Matthias T..(2014).Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis.PROTEIN SCIENCE,23(7),932-939. |
| MLA | Ma, Qingjun,et al."Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis".PROTEIN SCIENCE 23.7(2014):932-939. |
入库方式： OAI收割
来源：海洋研究所
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