Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis
文献类型：期刊论文
| 作者 | Cui, Jian dong1,2,3; Cui, Li li1,2; Zhang, Song ping2,4; Zhang, Yu fei2; Su, Zhi guo2; Ma, Guang hui2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-05-13 |
| 卷号 | 9期号:5页码:8 |
| 关键词 | BOVINE SERUM-ALBUMIN PENICILLIN-G ACYLASE ORGANIC MEDIA SILICA CLEAS IMMOBILIZATION BIOCATALYSTS STABILIZATION COAGGREGATION NANOPARTICLES |
| ISSN号 | 1932-6203 |
| 其他题名 | PLoS One |
| 中文摘要 | Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) indicated that PAL-CLEAs were inlayed in nanoparticle aggregates. The HM-PAL-CLEAs revealed a broader limit in optimal pH compared to free enzyme and PAL-CLEAs. Although there is no big difference in K-m of enzyme in CLEAs and HM-PAL-CLEAs, V-max of HM-PAL-CLEAs is about 1.75 times higher than that of CLEAs. Compared with free enzyme and PAL-CLEAs, the HM-PAL-CLEAs also exhibited the highest thermal stability, denaturant stability and storage stability. The HM-PAL-CLEAs retained 30% initial activity even after 11 cycles of reuse, whereas PAL-CLEAs retained 35% of its initial activity only after 7 cycles. These results indicated that hybrid magnetic CLEAs technology might be used as a feasible and efficient solution for improving properties of immobilized enzyme in industrial application. |
| 英文摘要 | Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) indicated that PAL-CLEAs were inlayed in nanoparticle aggregates. The HM-PAL-CLEAs revealed a broader limit in optimal pH compared to free enzyme and PAL-CLEAs. Although there is no big difference in K-m of enzyme in CLEAs and HM-PAL-CLEAs, V-max of HM-PAL-CLEAs is about 1.75 times higher than that of CLEAs. Compared with free enzyme and PAL-CLEAs, the HM-PAL-CLEAs also exhibited the highest thermal stability, denaturant stability and storage stability. The HM-PAL-CLEAs retained 30% initial activity even after 11 cycles of reuse, whereas PAL-CLEAs retained 35% of its initial activity only after 7 cycles. These results indicated that hybrid magnetic CLEAs technology might be used as a feasible and efficient solution for improving properties of immobilized enzyme in industrial application. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; PENICILLIN-G ACYLASE ; ORGANIC MEDIA ; SILICA ; CLEAS ; IMMOBILIZATION ; BIOCATALYSTS ; STABILIZATION ; COAGGREGATION ; NANOPARTICLES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000336369200072 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10892]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Tianjin Univ Sci & Technol, Key Lab Ind Microbiol, Tai Da Dev Area, Minist Educ, Tianjin, Peoples R China 4.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Jian dong,Cui, Li li,Zhang, Song ping,et al. Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis[J]. PLOS ONE,2014,9(5):8. |
| APA | Cui, Jian dong,Cui, Li li,Zhang, Song ping,Zhang, Yu fei,Su, Zhi guo,&Ma, Guang hui.(2014).Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis.PLOS ONE,9(5),8. |
| MLA | Cui, Jian dong,et al."Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis".PLOS ONE 9.5(2014):8. |
入库方式： OAI收割
来源：过程工程研究所
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