SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment
文献类型：期刊论文
| 作者 | Liu, Hongmei1,2; Li, Yan1,2; Mozhi, Anbu3; Zhang, Liang1,2; Liu, Yilan1,2; Xu, Xia1; Xing, Jianmin1; Liang, Xingjie3; Ma, Guanghui1; Yang, Jun1 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 35期号:24页码:6519-6533 |
| 关键词 | Combined effect siRNA-phospholipids conjugate Gene silencing Loading efficiency |
| ISSN号 | 0142-9612 |
| 其他题名 | Biomaterials |
| 中文摘要 | Due to low charge density and stiff backbone structure, small interfering RNA (siRNA) has inherently poor binding ability to cationic polymers and lipid carriers, which results in low siRNA loading efficiency and limits siRNA success in clinical application. Here, siRNA-phospholipids conjugates are developed, which integrate the characteristics of the two phospholipids to self-assemble via hydrophilic siRNA and hydrophobic phospholipid tails to overcome the siRNA's stiff backbone structures and enhance the siRNA loading efficiency. In this study, the thiol-modified sense and antisense siRNA are chemically conjugated with phospholipids to form sense and antisense siRNA-phospholipid, and then these sense or antisense siRNA-phospholipids with equal amounts are annealed to generate siRNA-phospholipids. The siRNA-phospholipids can serve dual functions as agents that can silence gene expression and as a component of nanoparticles to embed hydrophobic anticancer drugs to cure tumor. siRNA-phospholipids together with cationic lipids and DSPE-PEG2000 fuse around PLGA to form siRNA-phospholipids enveloped nanoparticles (siRNA-PCNPs), which can deliver siRNAs and hydrophobic anticancer drugs into tumor. In animal models, intravenously injected siRNA-PCNPs embedded DOX (siPlk1-PCNPs/DOX) is highly effective in inhibiting tumor growth. The results indicate that the siRNA-PCNPs can be potentially applied as a safe and efficient gene and anticancer drug delivery carrier. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Due to low charge density and stiff backbone structure, small interfering RNA (siRNA) has inherently poor binding ability to cationic polymers and lipid carriers, which results in low siRNA loading efficiency and limits siRNA success in clinical application. Here, siRNA-phospholipids conjugates are developed, which integrate the characteristics of the two phospholipids to self-assemble via hydrophilic siRNA and hydrophobic phospholipid tails to overcome the siRNA's stiff backbone structures and enhance the siRNA loading efficiency. In this study, the thiol-modified sense and antisense siRNA are chemically conjugated with phospholipids to form sense and antisense siRNA-phospholipid, and then these sense or antisense siRNA-phospholipids with equal amounts are annealed to generate siRNA-phospholipids. The siRNA-phospholipids can serve dual functions as agents that can silence gene expression and as a component of nanoparticles to embed hydrophobic anticancer drugs to cure tumor. siRNA-phospholipids together with cationic lipids and DSPE-PEG2000 fuse around PLGA to form siRNA-phospholipids enveloped nanoparticles (siRNA-PCNPs), which can deliver siRNAs and hydrophobic anticancer drugs into tumor. In animal models, intravenously injected siRNA-PCNPs embedded DOX (siPlk1-PCNPs/DOX) is highly effective in inhibiting tumor growth. The results indicate that the siRNA-PCNPs can be potentially applied as a safe and efficient gene and anticancer drug delivery carrier. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | POLO-LIKE KINASES ; CO-DELIVERY ; HYBRID NANOPARTICLES ; EFFICIENT ; POLYMER ; DOXORUBICIN ; MICELLES ; TRANSFECTION ; NANOCARRIERS ; CHEMOTHERAPY |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000338804500036 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10936]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongmei,Li, Yan,Mozhi, Anbu,et al. SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment[J]. BIOMATERIALS,2014,35(24):6519-6533. |
| APA | Liu, Hongmei.,Li, Yan.,Mozhi, Anbu.,Zhang, Liang.,Liu, Yilan.,...&Zhang, Xin.(2014).SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment.BIOMATERIALS,35(24),6519-6533. |
| MLA | Liu, Hongmei,et al."SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment".BIOMATERIALS 35.24(2014):6519-6533. |
入库方式： OAI收割
来源：过程工程研究所
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