Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products
文献类型：期刊论文
| 作者 | Qi, Benkun; Chen, Xiangrong; Yi, Su; Wan, Yinhua |
| 刊名 | ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 33期号:2页码:497-503 |
| 关键词 | acetone-butanol-ethanol cellulase enzymatic hydrolysis -glucosidase xylanase |
| ISSN号 | 1944-7442 |
| 其他题名 | Environ. Prog. Sustain. Energy |
| 中文摘要 | Simultaneous saccharification and fermentation has been regarded as a promising process for bioconversion of lignocellulosic biomass to acetone-butanol-ethanol (ABE); however, there exits potential inhibition of cellulase, -glucosidase, and xylanase by the fermentation product(s) due to the combination of enzymatic hydrolysis and microbial fermentation in one vessel. The effects of ABE fermentation products including acetone, butanol, ethanol, acetic acid, and butyric acid, individually and in a combination, on the cellulase, -glucosidase, and xylanase activities and enzymatic saccharification of dilute acid pretreated wheat straw (WS) were studied. The experimental results showed that butanol and mixture of inhibitor compounds strongly depressed cellulolytic and hemicellulolytic activities, and no synergistic inhibiting effect between inhibitors on enzyme activities was observed. Saccharification of pretreated WS was mainly affected by butanol and temperature. Moreover, preincubation of enzyme solutions with inhibitors mixture for 72 h decreased cellulase and xylanase activities by 15-25% at the three temperatures examined (30, 37, and 50 degrees C), and a significant decrease (64%) in -glucosidase activity was found at 50 degrees C. (c) 2013 American Institute of Chemical Engineers Environ Prog, 33: 497-503, 2014 |
| 英文摘要 | Simultaneous saccharification and fermentation has been regarded as a promising process for bioconversion of lignocellulosic biomass to acetone-butanol-ethanol (ABE); however, there exits potential inhibition of cellulase, -glucosidase, and xylanase by the fermentation product(s) due to the combination of enzymatic hydrolysis and microbial fermentation in one vessel. The effects of ABE fermentation products including acetone, butanol, ethanol, acetic acid, and butyric acid, individually and in a combination, on the cellulase, -glucosidase, and xylanase activities and enzymatic saccharification of dilute acid pretreated wheat straw (WS) were studied. The experimental results showed that butanol and mixture of inhibitor compounds strongly depressed cellulolytic and hemicellulolytic activities, and no synergistic inhibiting effect between inhibitors on enzyme activities was observed. Saccharification of pretreated WS was mainly affected by butanol and temperature. Moreover, preincubation of enzyme solutions with inhibitors mixture for 72 h decreased cellulase and xylanase activities by 15-25% at the three temperatures examined (30, 37, and 50 degrees C), and a significant decrease (64%) in -glucosidase activity was found at 50 degrees C. (c) 2013 American Institute of Chemical Engineers Environ Prog, 33: 497-503, 2014 |
| WOS标题词 | Science & Technology ; Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Engineering, Environmental ; Engineering, Chemical ; Engineering, Industrial ; Environmental Sciences |
| 研究领域[WOS] | Engineering ; Environmental Sciences & Ecology |
| 关键词[WOS] | SIMULTANEOUS SACCHARIFICATION ; CLOSTRIDIUM-BEIJERINCKII ; HARDWOOD ; BIOMASS ; BIOFUEL |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000334298800021 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10949]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Benkun,Chen, Xiangrong,Yi, Su,et al. Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products[J]. ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY,2014,33(2):497-503. |
| APA | Qi, Benkun,Chen, Xiangrong,Yi, Su,&Wan, Yinhua.(2014).Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products.ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY,33(2),497-503. |
| MLA | Qi, Benkun,et al."Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products".ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY 33.2(2014):497-503. |
入库方式： OAI收割
来源：过程工程研究所
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