Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation
文献类型：期刊论文
| 作者 | Qiu, Weihua; Zhang, Wenyan; Chen, Hongzhang |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 156期号:1页码:368-371 |
| 关键词 | Natural laccase mediator Water-washed solution Steam exploded corn straw Nanofiltration Successive organic solvent extraction |
| ISSN号 | 0960-8524 |
| 其他题名 | Bioresour. Technol. |
| 中文摘要 | Artificially synthetic mediators of laccase had the limitation of high cost and possible toxicity. The separation of natural laccase mediators from water-washed solution (WWS) of steam exploded corn straw (SECS) was studied using nano-filtration and successive organic solvents extraction. Results indicated that the UV absorption intensity of nano-filtrated WWS was significantly enhanced. The UV absorption intensity of each extractive from WWS could be ranked as ether extractive (EE) > ethyl acetate extractive (EAE) > chloroform extractive (CE). Decoloration of crystal violet catalyzed by laccase/EE was higher than that by laccase/ABTS, which was 66.95% and 61.9% at 8 h, respectively. All the decoloration rates of malachite green at 60 min using EE, EAE and ABTS as mediator were both more than 80%. This research would benefit for broaden the source of laccase mediator and reduce the using cost of laccase/mediator system. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Artificially synthetic mediators of laccase had the limitation of high cost and possible toxicity. The separation of natural laccase mediators from water-washed solution (WWS) of steam exploded corn straw (SECS) was studied using nano-filtration and successive organic solvents extraction. Results indicated that the UV absorption intensity of nano-filtrated WWS was significantly enhanced. The UV absorption intensity of each extractive from WWS could be ranked as ether extractive (EE) > ethyl acetate extractive (EAE) > chloroform extractive (CE). Decoloration of crystal violet catalyzed by laccase/EE was higher than that by laccase/ABTS, which was 66.95% and 61.9% at 8 h, respectively. All the decoloration rates of malachite green at 60 min using EE, EAE and ABTS as mediator were both more than 80%. This research would benefit for broaden the source of laccase mediator and reduce the using cost of laccase/mediator system. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | BIOETHANOL PRODUCTION ; WHEAT-STRAW ; DECOLORIZATION ; PULP ; DYES ; TRIPHENYLMETHANE ; LIGNOCELLULOSE ; DETOXIFICATION ; RECALCITRANT ; TECHNOLOGIES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000334828500051 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10953]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Weihua,Zhang, Wenyan,Chen, Hongzhang. Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation[J]. BIORESOURCE TECHNOLOGY,2014,156(1):368-371. |
| APA | Qiu, Weihua,Zhang, Wenyan,&Chen, Hongzhang.(2014).Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation.BIORESOURCE TECHNOLOGY,156(1),368-371. |
| MLA | Qiu, Weihua,et al."Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation".BIORESOURCE TECHNOLOGY 156.1(2014):368-371. |
入库方式： OAI收割
来源：过程工程研究所
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