Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover
文献类型：期刊论文
| 作者 | Wang, Ning1,2; Chen, Hong-Zhang1 |
| 刊名 | JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 8期号:3页码:308-316 |
| 关键词 | Biorefinery Corn Stover Hemicelluloses Steam Explosion 2 3-Butanediol |
| ISSN号 | 1556-6560 |
| 其他题名 | J. Biobased Mater. Bioenergy |
| 中文摘要 | Currently, the use of low-cost lignocellulosic biomass as an alternative substrate for 2,3-butanediol (2,3-BD) production to improve the economics of the process received considerable attention. But cellulose as the substrate required expensive cellulase, which increased the production costs. In this paper, hemicelluloses sugars were fractionated from corn stover by steam explosion. The objective of this study was to develop the corn stover hemicelluloses as carbon source to produce 2,3BD. After steam explosion, 69.81% of hemicelluloses were degraded to monosaccharide (glucose, xylose and arabinose) and oligosaccharide, meanwhile some by-products, such as acetic acid, furfural, 5-hydroxymethylfurfual (5-HMF) and soluble lignin compounds (SLC) were produced in the steam explosion process as well. Paenibacillus polymyxa S-07 could utilize all the hemicelluloses sugars to ferment 2,3-BD. Acetic acid could not only inhibit the production of by-products, but also promote the conversion of sugars to 2,3-BD. Compared with the fermentation without adding acetic acid, the concentration of 2,3-BD increased from 16.30 g/l to 22.41 g/l with adding 7 g/l acetic acid. Furfural and 5-HMF below 1 g/l promoted the production of 2,3-BD, and inhibited the production of 2,3-BD over 1 g/l. SLC was the inhibitor in the hemicelluloses extract. SLC over 2.62 g/l inhibited the growth of P. polymyxa S-07 severely. After detoxification of the extract with active carbon, SLC below 1.117 g/l almost had no effect on the production of 2,3-BD, the yield of 2,3-BD reached 12.07 g/l using the extract as carbon source, and the conversion rate of 2,3-BD was 0.42 g/g sugar. This study provides a potential feedstock for 2,3-butanediol production. |
| 英文摘要 | Currently, the use of low-cost lignocellulosic biomass as an alternative substrate for 2,3-butanediol (2,3-BD) production to improve the economics of the process received considerable attention. But cellulose as the substrate required expensive cellulase, which increased the production costs. In this paper, hemicelluloses sugars were fractionated from corn stover by steam explosion. The objective of this study was to develop the corn stover hemicelluloses as carbon source to produce 2,3BD. After steam explosion, 69.81% of hemicelluloses were degraded to monosaccharide (glucose, xylose and arabinose) and oligosaccharide, meanwhile some by-products, such as acetic acid, furfural, 5-hydroxymethylfurfual (5-HMF) and soluble lignin compounds (SLC) were produced in the steam explosion process as well. Paenibacillus polymyxa S-07 could utilize all the hemicelluloses sugars to ferment 2,3-BD. Acetic acid could not only inhibit the production of by-products, but also promote the conversion of sugars to 2,3-BD. Compared with the fermentation without adding acetic acid, the concentration of 2,3-BD increased from 16.30 g/l to 22.41 g/l with adding 7 g/l acetic acid. Furfural and 5-HMF below 1 g/l promoted the production of 2,3-BD, and inhibited the production of 2,3-BD over 1 g/l. SLC was the inhibitor in the hemicelluloses extract. SLC over 2.62 g/l inhibited the growth of P. polymyxa S-07 severely. After detoxification of the extract with active carbon, SLC below 1.117 g/l almost had no effect on the production of 2,3-BD, the yield of 2,3-BD reached 12.07 g/l using the extract as carbon source, and the conversion rate of 2,3-BD was 0.42 g/g sugar. This study provides a potential feedstock for 2,3-butanediol production. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials |
| 研究领域[WOS] | Chemistry ; Energy & Fuels ; Materials Science |
| 关键词[WOS] | ASSISTED BIOMASS FRACTIONATION ; WHEAT-STRAW ; KLEBSIELLA-PNEUMONIAE ; BACILLUS-POLYMYXA ; ACID ; BIOREFINERIES ; FERMENTATION ; TECHNOLOGIES ; BIOETHANOL ; EXPLOSION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000339422200004 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10981]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ning,Chen, Hong-Zhang. Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2014,8(3):308-316. |
| APA | Wang, Ning,&Chen, Hong-Zhang.(2014).Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,8(3),308-316. |
| MLA | Wang, Ning,et al."Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 8.3(2014):308-316. |
入库方式： OAI收割
来源：过程工程研究所
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