Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion
文献类型：期刊论文
| 作者 | Wang, Ziqiang; Wang, Yunshan; Shi, Hong; Su, Zhiguo |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 172期号:8页码:3989-4001 |
| 关键词 | L-(+)-Tartaric acid cis-Epoxysuccinate hydrolase Heterogeneous bioconversion Process optimization Scale-up |
| ISSN号 | 0273-2289 |
| 其他题名 | Appl. Biochem. Biotechnol. |
| 中文摘要 | An Escherichia coli-engineered bacterium with cis-epoxysuccinate hydrolase (ESH) activity was used to catalyze the stereospecific hydrolysis of cis-epoxysuccinic acid to l-(+)-tartaric acid. The effect of the substrate composition on the production efficiency of l-(+)-tartaric acid was investigated. Based on the sodium-type homogeneous substrate system, a heterogeneous substrate system, composed of 1.2 M sodium-type substrate and 1.8 M calcium-type substrate, was designed to improve ESH catalytic efficiency. After process optimization, a catalytic efficiency of 9.37 x 10(-3) g U-1 h(-1) was obtained with fed-batch mode in the heterogeneous substrate system, about a twofold increase compared to the traditional bioconversion process with Nocardia tartaricans cells. The scale-up tests were carried out in a 15-m(3) stirred tank reactor, which indicated that the heterogeneous substrate system had great application prospect for the l-(+)-tartaric acid industrial production. |
| 英文摘要 | An Escherichia coli-engineered bacterium with cis-epoxysuccinate hydrolase (ESH) activity was used to catalyze the stereospecific hydrolysis of cis-epoxysuccinic acid to l-(+)-tartaric acid. The effect of the substrate composition on the production efficiency of l-(+)-tartaric acid was investigated. Based on the sodium-type homogeneous substrate system, a heterogeneous substrate system, composed of 1.2 M sodium-type substrate and 1.8 M calcium-type substrate, was designed to improve ESH catalytic efficiency. After process optimization, a catalytic efficiency of 9.37 x 10(-3) g U-1 h(-1) was obtained with fed-batch mode in the heterogeneous substrate system, about a twofold increase compared to the traditional bioconversion process with Nocardia tartaricans cells. The scale-up tests were carried out in a 15-m(3) stirred tank reactor, which indicated that the heterogeneous substrate system had great application prospect for the l-(+)-tartaric acid industrial production. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CIS-EPOXYSUCCINATE HYDROLASE ; PECTATE GEL BEADS ; EPOXIDE HYDROLASE ; NOCARDIA-TARTARICANS ; ESCHERICHIA-COLI ; EXPRESSION ; PURIFICATION ; ENTRAPMENT ; CULTURE ; CLONING |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000334433200022 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10989]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ziqiang,Wang, Yunshan,Shi, Hong,et al. Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2014,172(8):3989-4001. |
| APA | Wang, Ziqiang,Wang, Yunshan,Shi, Hong,&Su, Zhiguo.(2014).Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,172(8),3989-4001. |
| MLA | Wang, Ziqiang,et al."Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 172.8(2014):3989-4001. |
入库方式： OAI收割
来源：过程工程研究所
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