Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine
文献类型：期刊论文
| 作者 | Wei, Xuetuan1,2; Luo, Mingfang3; Liu, Huizhou2 |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 116期号:1页码:418-423 |
| 关键词 | Nattokinase Nanosilver Antithrombotic Antimicrobial Coating |
| ISSN号 | 0927-7765 |
| 其他题名 | Colloid Surf. B-Biointerfaces |
| 中文摘要 | The bifunctional coating with antithrombotic and antimicrobial activity was developed using nattokinase (NK) and nanosilver (AgNPs). Firstly, the adsorption interactions between NK and AgNPs were confirmed, and the composite particles of NK-AgNPs were prepared by adsorption of NK with AgNPs. At 5 FU/mL of NK concentration, the saturation adsorption capacity reached 24.35 FU/mg AgNPs with a high activity recovery of 97%, and adsorption by AgNPs also enhanced the heat stability and anticoagulant effect of NK. Based on the electrostatic force driven layer-by-layer self-assembly, the NK-AgNPs were further assembled with polyethylenimine (PEI) to form coating. UV-vis analysis showed that the self-assembly process was regular, and atom force microscopy analysis indicated that NK-AgNPs were uniformly embedded into the coating. The NK-AgNPs-PEI composite coating showed potent antithrombotic activity and antibacterial activity. This study developed a novel strategy to construct the bifunctional coating with antithrombotic and antimicrobial properties, and the coating material showed promising potential to be applied in the medical device. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | The bifunctional coating with antithrombotic and antimicrobial activity was developed using nattokinase (NK) and nanosilver (AgNPs). Firstly, the adsorption interactions between NK and AgNPs were confirmed, and the composite particles of NK-AgNPs were prepared by adsorption of NK with AgNPs. At 5 FU/mL of NK concentration, the saturation adsorption capacity reached 24.35 FU/mg AgNPs with a high activity recovery of 97%, and adsorption by AgNPs also enhanced the heat stability and anticoagulant effect of NK. Based on the electrostatic force driven layer-by-layer self-assembly, the NK-AgNPs were further assembled with polyethylenimine (PEI) to form coating. UV-vis analysis showed that the self-assembly process was regular, and atom force microscopy analysis indicated that NK-AgNPs were uniformly embedded into the coating. The NK-AgNPs-PEI composite coating showed potent antithrombotic activity and antibacterial activity. This study developed a novel strategy to construct the bifunctional coating with antithrombotic and antimicrobial properties, and the coating material showed promising potential to be applied in the medical device. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biophysics ; Chemistry ; Materials Science |
| 关键词[WOS] | HYDROPHILIC SURFACE-COATINGS ; SILVER NANOPARTICLES ; HEPARIN ; FOOD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000335706100055 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10990]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Huazhong Agr Univ, Coll Food Sci & Technol, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Technol Transfer Ctr, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Xuetuan,Luo, Mingfang,Liu, Huizhou. Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2014,116(1):418-423. |
| APA | Wei, Xuetuan,Luo, Mingfang,&Liu, Huizhou.(2014).Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine.COLLOIDS AND SURFACES B-BIOINTERFACES,116(1),418-423. |
| MLA | Wei, Xuetuan,et al."Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine".COLLOIDS AND SURFACES B-BIOINTERFACES 116.1(2014):418-423. |
入库方式： OAI收割
来源：过程工程研究所
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