Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells
文献类型：期刊论文
| 作者 | Wei, Yuping1,2; Li, Chunlong1,2; Zhang, Liang1,2; Xu, Xia1 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 1838期号:7页码:1911-1920 |
| 关键词 | Cell penetrating peptide Trehalose Stabilization Lyophilization Mammalian cell |
| ISSN号 | 0005-2736 |
| 其他题名 | Biochim. Biophys. Acta-Biomembr. |
| 中文摘要 | Stabilization of cells in a desiccated state can significantly simplify the storage and transportation and save expenses for clinical applications. Introduction of the impermeable disaccharide, trehalose, into cells is an important step to improve the desiccation tolerance of cells. In this study, a novel cell penetrating peptide, KRKRWHW, was developed based on molecular simulations. The peptide exhibited little cytotoxicity and high penetrating efficiency into mammalian cells. The cell viability of mouse embryonic fibroblasts (MEFs) after the incubation with various concentrations of KRKRWHW from 0.01 mM to 5 mM at 37 degrees C for 4 h was maintained at around 100%. The peptide was able to penetrate into MEFs within 1 h at 37 degrees C with an efficiency of around 90% at 0.1 mM. Trehalose, as a cargo coupled with the peptide of KRKRWHW through hydrogen bond and pi-pi bond, was successfully loaded into the MEFs. This novel peptide provides a novel approach for the delivery of trehalose into mammalian cells. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | Stabilization of cells in a desiccated state can significantly simplify the storage and transportation and save expenses for clinical applications. Introduction of the impermeable disaccharide, trehalose, into cells is an important step to improve the desiccation tolerance of cells. In this study, a novel cell penetrating peptide, KRKRWHW, was developed based on molecular simulations. The peptide exhibited little cytotoxicity and high penetrating efficiency into mammalian cells. The cell viability of mouse embryonic fibroblasts (MEFs) after the incubation with various concentrations of KRKRWHW from 0.01 mM to 5 mM at 37 degrees C for 4 h was maintained at around 100%. The peptide was able to penetrate into MEFs within 1 h at 37 degrees C with an efficiency of around 90% at 0.1 mM. Trehalose, as a cargo coupled with the peptide of KRKRWHW through hydrogen bond and pi-pi bond, was successfully loaded into the MEFs. This novel peptide provides a novel approach for the delivery of trehalose into mammalian cells. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MOLECULAR-DYNAMICS SIMULATION ; HIV-1 TAT PROTEIN ; PLASMA-MEMBRANE ; IN-VITRO ; INTRACELLULAR TREHALOSE ; DESICCATION TOLERANCE ; DRY STATE ; STABILIZATION ; ACID ; VIVO |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000336695300026 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/10992]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Yuping,Li, Chunlong,Zhang, Liang,et al. Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2014,1838(7):1911-1920. |
| APA | Wei, Yuping,Li, Chunlong,Zhang, Liang,&Xu, Xia.(2014).Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1838(7),1911-1920. |
| MLA | Wei, Yuping,et al."Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1838.7(2014):1911-1920. |
入库方式： OAI收割
来源：过程工程研究所
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