Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol
文献类型：期刊论文
| 作者 | Xu, Yanqing1,2; Zhang, Liang1,2; Xu, Jiandong1; Wei, Yuping1,2; Xu, Xia1 |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2014-05-15 |
| 卷号 | 550期号:1页码:67-76 |
| 关键词 | Human embryonic stem cells Human induced pluripotent stem cells Cryoprotectant permeability Hydraulic permeability Cryopreservation |
| ISSN号 | 0003-9861 |
| 其他题名 | Arch. Biochem. Biophys. |
| 中文摘要 | Due to the unlimited capacity of self-renew and ability to differentiate into derivatives of three germ layers, human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), have a great potential in regenerative medicine. A major challenge we are facing during the long-term storage of human pluripotent stem cells with the conventional slow cooling rate is the low cell recovery rate after cryopreservation which cannot meet the requirements for the future clinical applications. Evaluating the cell membrane permeability and the corresponding activation energy of hESCs and hiPSCs for water and different cryoprotective agents (CPA), including dimethyl sulfoxide (Me2SO), 1,2-propandiol and glycerol, is important for facilitating the development of cryopreservation protocol to enhance cell recovery after the cryopreservation. The osmotically inactive volume of hESCs and hiPSCs determined using the Boyle-van't Hoff model was 0.32V(0) and 0.42V(0), respectively. The membrane permeability was assessed from the volume changes of cells exposed to Me2SO, 1,2-propanediol and glycerol at the temperatures ranging from 8 to 30 degrees C. These results showed the biophysical differences between hESCs and hiPSCs. Their activation energy for water and CPAs extrapolated from the Arrhenius relationship indicated that the water transport was probably not through the channel-mediated mechanism. (C) 2014 Elsevier Inc. All rights reserved. |
| 英文摘要 | Due to the unlimited capacity of self-renew and ability to differentiate into derivatives of three germ layers, human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), have a great potential in regenerative medicine. A major challenge we are facing during the long-term storage of human pluripotent stem cells with the conventional slow cooling rate is the low cell recovery rate after cryopreservation which cannot meet the requirements for the future clinical applications. Evaluating the cell membrane permeability and the corresponding activation energy of hESCs and hiPSCs for water and different cryoprotective agents (CPA), including dimethyl sulfoxide (Me2SO), 1,2-propandiol and glycerol, is important for facilitating the development of cryopreservation protocol to enhance cell recovery after the cryopreservation. The osmotically inactive volume of hESCs and hiPSCs determined using the Boyle-van't Hoff model was 0.32V(0) and 0.42V(0), respectively. The membrane permeability was assessed from the volume changes of cells exposed to Me2SO, 1,2-propanediol and glycerol at the temperatures ranging from 8 to 30 degrees C. These results showed the biophysical differences between hESCs and hiPSCs. Their activation energy for water and CPAs extrapolated from the Arrhenius relationship indicated that the water transport was probably not through the channel-mediated mechanism. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RATIONALLY OPTIMIZED CRYOPRESERVATION ; INTRACELLULAR ICE FORMATION ; FREEZING-INJURY ; ROCK INHIBITOR ; HUMAN OOCYTES ; WATER ; KINETICS ; LINES ; DIMETHYLSULFOXIDE ; HYPOTHESIS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000336780300009 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11002]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yanqing,Zhang, Liang,Xu, Jiandong,et al. Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2014,550(1):67-76. |
| APA | Xu, Yanqing,Zhang, Liang,Xu, Jiandong,Wei, Yuping,&Xu, Xia.(2014).Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,550(1),67-76. |
| MLA | Xu, Yanqing,et al."Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 550.1(2014):67-76. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。