Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase
文献类型：期刊论文
| 作者 | Xue, Xiaoying1,2; Ji, Shaoyang1; Mu, Qimeng1,2; Hu, Tao1 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 49期号:7页码:1092-1096 |
| 关键词 | Polyethylene glycol Staphylokinase Heat treatment Bioactivity |
| ISSN号 | 1359-5113 |
| 其他题名 | Process Biochem. |
| 中文摘要 | PEGylation can effectively improve the therapeutic potential of staphylokinase (SAK), a thrombolysis agent for therapy of myocardial infarction. However, polyethylene glycol (PEG) can sterically shield SAK and drastically decrease its bioactivity. In the present study, N-terminally PEGylated SAKs.(5 and 20 kDa PEG), C-terminally PEGylated SAKs with phenyl linker and the ones with amyl linker (5 and 20 kDa PEG) were prepared. The effects of the PEG length, the PEGylation site and linker chemistry on the bioactivity of the heat-treated PEGylated SAK were investigated. Heat treatment at 70 C for 2 h can improve the bioactivity of the C-terminally PEGylated SAKs, where the one with amyl linker and 20 kDa PEG showed the highest increase extent (27%) in the bioactivity. Thus, our study can advance the development of long-acting pharmaceutical protein with high bioactivity. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | PEGylation can effectively improve the therapeutic potential of staphylokinase (SAK), a thrombolysis agent for therapy of myocardial infarction. However, polyethylene glycol (PEG) can sterically shield SAK and drastically decrease its bioactivity. In the present study, N-terminally PEGylated SAKs.(5 and 20 kDa PEG), C-terminally PEGylated SAKs with phenyl linker and the ones with amyl linker (5 and 20 kDa PEG) were prepared. The effects of the PEG length, the PEGylation site and linker chemistry on the bioactivity of the heat-treated PEGylated SAK were investigated. Heat treatment at 70 C for 2 h can improve the bioactivity of the C-terminally PEGylated SAKs, where the one with amyl linker and 20 kDa PEG showed the highest increase extent (27%) in the bioactivity. Thus, our study can advance the development of long-acting pharmaceutical protein with high bioactivity. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | DRUG-DELIVERY ; STABILITY ; PROTEINS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000338605500005 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11003]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Xiaoying,Ji, Shaoyang,Mu, Qimeng,et al. Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase[J]. PROCESS BIOCHEMISTRY,2014,49(7):1092-1096. |
| APA | Xue, Xiaoying,Ji, Shaoyang,Mu, Qimeng,&Hu, Tao.(2014).Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase.PROCESS BIOCHEMISTRY,49(7),1092-1096. |
| MLA | Xue, Xiaoying,et al."Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase".PROCESS BIOCHEMISTRY 49.7(2014):1092-1096. |
入库方式： OAI收割
来源：过程工程研究所
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