Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value
文献类型：期刊论文
| 作者 | Yan, Daojiang1,2; Wang, Caixia1,2; Zhou, Jiemin1,2; Liu, Yilan1,2; Yang, Maohua1; Xing, Jianmin1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 156期号:1页码:232-239 |
| 关键词 | Succinic acid Reductive pathway Reducing power Low pH Saccharomyces cerevisiae |
| ISSN号 | 0960-8524 |
| 其他题名 | Bioresour. Technol. |
| 中文摘要 | Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 +/- 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 +/- 0.28 g/L by the deletion of GPD1 and even higher to 9.98 +/- 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 +/- 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 +/- 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 +/- 0.28 g/L by the deletion of GPD1 and even higher to 9.98 +/- 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 +/- 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | CHEMOSTAT CULTURES ; ESCHERICHIA-COLI ; PYRUVATE ; GENE ; ISOENZYMES ; EVOLUTION ; FUMARASE ; GLUCOSE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000334828500032 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11004]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Daojiang,Wang, Caixia,Zhou, Jiemin,et al. Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value[J]. BIORESOURCE TECHNOLOGY,2014,156(1):232-239. |
| APA | Yan, Daojiang,Wang, Caixia,Zhou, Jiemin,Liu, Yilan,Yang, Maohua,&Xing, Jianmin.(2014).Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value.BIORESOURCE TECHNOLOGY,156(1),232-239. |
| MLA | Yan, Daojiang,et al."Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value".BIORESOURCE TECHNOLOGY 156.1(2014):232-239. |
入库方式： OAI收割
来源：过程工程研究所
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