On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures
文献类型：期刊论文
| 作者 | Zhang, Dongmei1,2; Yan, Fei1,2; Sun, Zhongliang1,2; Zhang, Qinghua1; Xue, Shengzhang1; Cong, Wei1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 164期号:1页码:86-92 |
| 关键词 | Microalgae Carbon metabolism Energy metabolism On-line monitor Biomass composition |
| ISSN号 | 0960-8524 |
| 其他题名 | Bioresour. Technol. |
| 中文摘要 | In this study, a photobioreactor cultivation system and a calculation method for on-line monitoring of carbon and energy metabolism of microalgae were developed using Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures. Only 30-60% of carbon fixed in Calvin cycle was used for biomass and the rest was lost in light respiration. The net fixed carbon was assumed to be incorporated into protein, lipids, carbohydrates, and nucleic acids, whose contents calculated on-line fitted well with the experimental measurements. Intracellular ATPs were quantitatively divided for biomass production and cell maintenance, and the result is in accordance with known reports. Due to light limitation induced by high cell concentration in batch cultures, the proportion of CO2 loss in light respiration and the proportion of energy for maintenance rapidly increased in culturing process. Nitrogen starvation reduced the light respiration, thus decreasing CO2 loss and maintenance energy, but no effect on ATP requirement for cell growth. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | In this study, a photobioreactor cultivation system and a calculation method for on-line monitoring of carbon and energy metabolism of microalgae were developed using Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures. Only 30-60% of carbon fixed in Calvin cycle was used for biomass and the rest was lost in light respiration. The net fixed carbon was assumed to be incorporated into protein, lipids, carbohydrates, and nucleic acids, whose contents calculated on-line fitted well with the experimental measurements. Intracellular ATPs were quantitatively divided for biomass production and cell maintenance, and the result is in accordance with known reports. Due to light limitation induced by high cell concentration in batch cultures, the proportion of CO2 loss in light respiration and the proportion of energy for maintenance rapidly increased in culturing process. Nitrogen starvation reduced the light respiration, thus decreasing CO2 loss and maintenance energy, but no effect on ATP requirement for cell growth. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; LIGHT-INTENSITY ; GROWTH-RATES ; BIOMASS ; PHOTOBIOREACTOR ; MICROALGAE ; TEMPERATURE ; STARVATION ; NUTRIENTS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000338710500013 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11025]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dongmei,Yan, Fei,Sun, Zhongliang,et al. On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures[J]. BIORESOURCE TECHNOLOGY,2014,164(1):86-92. |
| APA | Zhang, Dongmei,Yan, Fei,Sun, Zhongliang,Zhang, Qinghua,Xue, Shengzhang,&Cong, Wei.(2014).On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures.BIORESOURCE TECHNOLOGY,164(1),86-92. |
| MLA | Zhang, Dongmei,et al."On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures".BIORESOURCE TECHNOLOGY 164.1(2014):86-92. |
入库方式： OAI收割
来源：过程工程研究所
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