Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography
文献类型：期刊论文
| 作者 | Zhang, Rongyue; Li, Qiang; Gao, Yang; Li, Juan; Huang, Yongdong; Song, Cui; Zhou, Weiqing; Ma, Guanghui; Su, Zhiguo |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2014-05-23 |
| 卷号 | 1343期号:1页码:109-118 |
| 关键词 | Hydrophilicity Gigaporous microspheres Anion-exchange medium Branched polyethyleneimine High-throughput |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | High hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | High hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | TRANSFER RADICAL POLYMERIZATION ; MICELLES SWELLING METHOD ; HIGH-CAPACITY ; CAPILLARY ELECTROCHROMATOGRAPHY ; POLY(GLYCIDYL METHACRYLATE) ; PERFUSION CHROMATOGRAPHY ; POLYSTYRENE MICROSPHERES ; LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; RAPID SEPARATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000336195800014 |
| 公开日期 | 2014-08-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11032]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Rongyue,Li, Qiang,Gao, Yang,et al. Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1343(1):109-118. |
| APA | Zhang, Rongyue.,Li, Qiang.,Gao, Yang.,Li, Juan.,Huang, Yongdong.,...&Su, Zhiguo.(2014).Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography.JOURNAL OF CHROMATOGRAPHY A,1343(1),109-118. |
| MLA | Zhang, Rongyue,et al."Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography".JOURNAL OF CHROMATOGRAPHY A 1343.1(2014):109-118. |
入库方式： OAI收割
来源：过程工程研究所
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