Enzymatic Hydrolysis of Penicillin in an Integrated System Containing Ionic Liquids
文献类型：会议论文
| 作者 | Jiang, Y. Y.; Guo, C.; Xia, H. S.; Liu, H. Z. |
| 出版日期 | 2010 |
| 会议名称 | 236th National Meeting of the American-Chemical-Society |
| 会议日期 | AUG 17-21, 2008 |
| 会议地点 | Philadelphia, PA |
| 关键词 | POTASSIUM PHOSPHATE SOLUTIONS ACYLASE-CATALYZED SYNTHESIS BETA-LACTAM ANTIBIOTICS 6-AMINOPENICILLANIC ACID POLY(ETHYLENE GLYCOL) ORGANIC COSOLVENTS WATER ACTIVITY SALT-SOLUTIONS LOW PH EQUILIBRIUM |
| 页码 | 77-89 |
| 其他题名 | ACS Symp. Ser. |
| 中文摘要 | Ionic liquids were employed as novel green solvents to develop an integrated process involving ionic liquids aqueous two-phase system (ILATPS) and mixed ionic liquids/water two-phase system (MILWS) for separation and enzymatic catalysis of penicillin. First, hydrophilic [C(4)mim]BF4 (1-butyl-3-methylimidazolium tetrafluoroborate) and NaH2PO4 salt form an ionic liquid aqueous two-phase system (ILATPS), which could extract penicillin from its fermentation broth efficiently. Second, hydrophobic [C(4)mim]PF6 (1-butyl-3-methylimidazolium hexafluorophosphate) is introduced into the ionic liquid-rich phase of ILATPS containing penicillin and converses it into MILWS. Penicillin is hydrolyzed by penicillin acylase in the water phase of MILWS at pH 5. The byproduct phenylacetic acid (PAA) is partitioned into the ionic liquids mixture phase, while the intended product 6-aminopenicillanic acid (6-APA) is precipitated at this pH. In comparison with a similar butyl acetate/water system (BAWS) at pH 4, MILWS exhibits two advantages: (1) The selectivity between PAA and penicillin is greatly optimized at pH 5 by varying the mole ratio of [C(4)mim]PF6/[C(4)mim]BF4 in MILWS, whereas in BAWS the unalterable nature of the organic solvent restricts the optimized pH for maximum selectivity between PAA and penicillin at pH 4. (2) The pH for 6-APA precipitation in BAWS is 4, whereas it shifts to pH 5 in MILWS due to the complexation between negatively charged 6-APA and the cationic surface of the ionic liquids micelle. As a result, the removal of the two products from the enzyme sphere at relatively high pH is permitted in MILWS, which is beneficial for enzymatic activity and stability in comparison with the acidic pH 4 environment in BAWS. |
| 收录类别 | ISTP |
| 会议网址 | |
| 会议录 | ACS Symposium Series 卷: 1038 页: 77-89
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| 会议录出版者 | Amer Chemical Soc |
| 语种 | 英语 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11133]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 |
Jiang, Y. Y.,Guo, C.,Xia, H. S.,et al. Enzymatic Hydrolysis of Penicillin in an Integrated System Containing Ionic Liquids[C]. 见:236th National Meeting of the American-Chemical-Society. Philadelphia, PA. AUG 17-21, 2008. |
入库方式： OAI收割
来源：过程工程研究所
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