Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1
文献类型：会议论文
| 作者 | Qiao, C. L. ; Shen, B. C. ; Xing, J. M. ; Huang, J. ; Zhang, J. L. ; Zhao, D. X. ; Yang, B. |
| 出版日期 | 2006 |
| 关键词 | high-density culture E coli carboxylesterase B1 recombinant gene expression HIGH-CELL-DENSITY FED-BATCH CULTURES GROWTH CULTIVATION POLLUTANTS GLUCOSE |
| 期号 | 2 |
| 页码 | 77-81 |
| 其他题名 | Int. Biodeterior. Biodegrad. |
| 中文摘要 | High-density culture was achieved through controlling specific growth rate by limiting glucose concentration to < 0.2gL(-1). Carboxylesterase B I capable of hydrolyzing organophosphate esters was purified from Escherichia coli strain BL21 carrying a cloned esterase B I gene from mosquito. The recombinant strain BL21 carrying pET-ESTB1 was used for the fermentation. Product formation was induced by either a temperature shift from 30 to 42 degrees C or by feeding a mixture of glucose and lactose. Cell growth and production of detoxifying enzyme were affected by oxygen availability. The maximum biomass of E. coli BL21 (pET-ESTB1) increased from 14.9 to 31.5 g dry cell weight l(-1). Using the host strain E. coli BL21 (DE3), detoxifying enzyme was over-expressed at a biomass level of up to 31.5 g dry weight l(-1). The enzyme had a molecular mass of 64 kDa, its optimum temperature was approx. 37 degrees C; at pH 7 the relative activity after 3 h was 85.9% at 28 degrees C, 64.9% at 34 degrees C, and 4.5% at 40 degrees C. The enzyme activity of cells grown at lower temperatures was much higher; at 18 degrees C it almost twice than at 20 or 22 degrees C. degrees 2006 Elsevier Ltd. All rights reserved. |
| 会议网址 | |
| 会议录 | International Biodeterioration & Biodegradation
 |
| 语种 | 英语 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11205]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 |
Qiao, C. L.,Shen, B. C.,Xing, J. M.,et al. Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1[C]. 见:. |
入库方式： OAI收割
来源：过程工程研究所
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