Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
文献类型：期刊论文
| 作者 | Zhong, Weiliang1; Zhang, Weiguo1; Wang, Shouyu1; Qin, Jianhua2 |
| 刊名 | plos one
 |
| 出版日期 | 2013-04-18 |
| 卷号 | 8期号:4页码:61283 |
| ISSN号 | 1932-6203 |
| 产权排序 | 待补充 |
| 通讯作者 | weiguo zhang |
| 合作状况 | 英 |
| 英文摘要 | in native fibrocartilage, mechanotransduction allows the cells to perceive the physical microenvironment not only through topographical cues from the extracellular matrix, but also through mechanical cues, such as interstitial flow. to create a microenvironment that simultaneously integrates nanotopography and flow stimulus, we developed a biomimetic microfluidic device embedded with aligned nanofibers to contain microchambers of different angles, which enabled the flow direction to form different angles with the fibers. using this device, we investigated the effects of microfluidic and nanotopographical environment on the morphology and fibrochondrogenesis of mesenchymal stem cells (mscs) and the involvement of rhoa/rock pathway and yes-associated protein (yap)/transcriptional co-activator with pdz-binding motif (taz). the results showed that the flow direction perpendicular to aligned nanofibers was conducive to fibrochondrogenesis of mscs. in addition, rock inhibitor and knockdown of yap/taz disrupted fibrochondrogenic differentiation of mscs. in conclusion, our data suggest the crucial role of mechanotransduction in regulating fibrochondrogenic differentiation of mscs, which may be mediated by rhoa/rock pathway and yap/taz. |
| WOS标题词 | science & technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | gene-expression ; chondrogenic differentiation ; focal adhesion ; nuclear shape ; fate ; microenvironment ; modulation ; pathway ; regeneration ; chondrocytes |
| 收录类别 | SCI |
| 资助信息 | 2，4 |
| 原文出处 | 61283 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317908700019 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119086]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Affiliated Hosp 1, Dept Orthopaed, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Weiliang,Zhang, Weiguo,Wang, Shouyu,et al. Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds[J]. plos one,2013,8(4):61283. |
| APA | Zhong, Weiliang,Zhang, Weiguo,Wang, Shouyu,&Qin, Jianhua.(2013).Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds.plos one,8(4),61283. |
| MLA | Zhong, Weiliang,et al."Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds".plos one 8.4(2013):61283. |
入库方式： OAI收割
来源：大连化学物理研究所
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