Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
文献类型：期刊论文
| 作者 | Li, Qinran1,2; Yang, Kaiguang1; Liu, Jinxiang1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | microchimica acta
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 180期号:15-16页码:1379-1386 |
| 关键词 | Hierarchical imprinting Protein imprinted polymers Transferrin. Selective recognition |
| 产权排序 | 待补充 |
| 通讯作者 | 张丽华 |
| 合作状况 | 英 |
| 英文摘要 | a novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. first, transferrin was immobilized on silica beads by non-covalent absorption. then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 a degrees c. finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. the binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. the imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease b, cytochrome c and beta-lactoglobulin. the results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. they may be applied as an artificial antibody to remove the high abundance proteins in plasma. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrostatic functional-groups ; enzyme-inhibitors ; ribonuclease-a ; polymers ; polyacrylamide ; plasma ; proteomics ; hydrogel ; beads ; sds |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 1386 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326803800004 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119109]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,et al. Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique[J]. microchimica acta,2013,180(15-16):1379-1386. |
| APA | Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2013).Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique.microchimica acta,180(15-16),1379-1386. |
| MLA | Li, Qinran,et al."Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique".microchimica acta 180.15-16(2013):1379-1386. |
入库方式： OAI收割
来源：大连化学物理研究所
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