Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns
文献类型：期刊论文
| 作者 | Liu, Zheyi1; Ou, Junjie1; Liu, Zhongshan1,2; Liu, Jing1,2; Lin, Hui1,2; Wang, Fangjun1; Zou, Hanfa1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2013-11-22 |
| 卷号 | 1317页码:138-147 |
| 关键词 | Hybrid monolithic capillary column Intact protein Reversed phase liquid chromatography High speed separation |
| ISSN号 | 0021-9673 |
| 产权排序 | 待补充 |
| 通讯作者 | 王方军 ; 邹汉法 |
| 合作状况 | 英 |
| 英文摘要 | high-efficient separation of intact proteins is still a huge challenge in proteome analysis of complex biological samples by using capillary columns. in this study, four foss-based hybrid monolithic capillary columns were prepared and applied in nano-flow liquid chromatography (nano-lc) separation of intact proteins. it was observed that the poss-based hybrid monolithic columns exhibit high permeability, good lc separation reproducibility and column efficiency for intact protein separation. the effects of different lc separation conditions such as flow rate, gradient steepness, column length and mobile phase additives on the lc separation efficiency of the poss-based hybrid monolithic column were systematically examined. finally, fast lc separation of 7 proteins mixture was realized in 2.5 min by using the optimized conditions on the 100 mu m i.d. poss-based hybrid monolithic capillary column. (c) 2013 published by elsevier b.v. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | liquid-chromatographic separation ; spectrometry-based proteomics ; top-down proteomics ; on-plate digestion ; mass-spectrometry ; quantitative-analysis ; stationary phases ; gradient-elution ; peak-capacity ; identification |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 147 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327229600016 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119129]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zheyi,Ou, Junjie,Liu, Zhongshan,et al. Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns[J]. journal of chromatography a,2013,1317:138-147. |
| APA | Liu, Zheyi.,Ou, Junjie.,Liu, Zhongshan.,Liu, Jing.,Lin, Hui.,...&Zou, Hanfa.(2013).Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns.journal of chromatography a,1317,138-147. |
| MLA | Liu, Zheyi,et al."Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns".journal of chromatography a 1317(2013):138-147. |
入库方式： OAI收割
来源：大连化学物理研究所
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