High throughput generation and trapping of individual agarose microgel using microfluidic approach
文献类型：期刊论文
| 作者 | Shi, Yang1; Gao, Xinghua1; Chen, Longqing2; Zhang, Min1; Ma, Jingyun1; Zhang, Xixiang2; Qin, Jianhua1 |
| 刊名 | microfluidics and nanofluidics
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 15期号:4页码:467-474 |
| 关键词 | Agarose Microgel 3D cell culture Microfluidic Droplet |
| ISSN号 | 1613-4982 |
| 产权排序 | 待补充 |
| 通讯作者 | 秦建华 |
| 合作状况 | 英 |
| 英文摘要 | microgel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. however, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-d cell culture in real-time. the established system was mainly composed of droplet generators with flow focusing t-junction and a series of array individual trap structures. the whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. the performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (accm) cells in the gelated agarose microgels. this established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3d matrix in real-time. it can also be extended for applications in the area of material synthesis and tissue engineering. |
| WOS标题词 | science & technology ; technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | nanoscience & nanotechnology ; instruments & instrumentation ; physics, fluids & plasmas |
| 研究领域[WOS] | science & technology - other topics ; instruments & instrumentation ; physics |
| 关键词[WOS] | drug-delivery ; microparticles ; particles ; cells ; flow ; gel |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 474 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324646000003 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119167]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.King Abdullah Univ Sci & Technol, Adv Nanofabricat Imaging & Characterizat Core Lab, Thuwal 239556900, Saudi Arabia |
| 推荐引用方式 GB/T 7714 | Shi, Yang,Gao, Xinghua,Chen, Longqing,et al. High throughput generation and trapping of individual agarose microgel using microfluidic approach[J]. microfluidics and nanofluidics,2013,15(4):467-474. |
| APA | Shi, Yang.,Gao, Xinghua.,Chen, Longqing.,Zhang, Min.,Ma, Jingyun.,...&Qin, Jianhua.(2013).High throughput generation and trapping of individual agarose microgel using microfluidic approach.microfluidics and nanofluidics,15(4),467-474. |
| MLA | Shi, Yang,et al."High throughput generation and trapping of individual agarose microgel using microfluidic approach".microfluidics and nanofluidics 15.4(2013):467-474. |
入库方式： OAI收割
来源：大连化学物理研究所
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