Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings
文献类型：期刊论文
| 作者 | Yuan, Yue1; Zhang, Jia3; Wang, Mengjing1; Mei, Bin1; Guan, Yafeng2; Liang, Gaolin1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013-02-05 |
| 卷号 | 85期号:3页码:1280-1284 |
| 产权排序 | 待补充 |
| 通讯作者 | gaolin liang |
| 合作状况 | 英 |
| 英文摘要 | taking advantage of a reduction-controlled biocompatible condensation reaction and self-assembly, we have developed a new method for the determination of glutathione (gsh) concentration in vitro and in hepg(2) human liver cancer cells. upon reduction by gsh under physiological conditions (ph 7.4 in buffer), the small molecule cbt-cys(set) condenses and self-assembles into nanorings, increasing the uv absorbance at 380 nm (with significant linear correlation in the 0-87 mu m gsh range and a limit of detection of 1 mu m). this method is also selective to gsh rather than cysteine in biological samples. through the use of added internal standards, we successfully determined the concentration of gsh in hepg2 cells to be 14.96 mu m (2.99 fmol/cell). to better understand the mechanism of nanoring self-assembly, the condensation product of cbt-cys(set) formed using different concentrations of gsh and different reaction times were characterized by transmission electron microscopy (tem). |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | biocompatible condensation reaction ; thiols ; disulfides |
| 收录类别 | SCI |
| 资助信息 | 2，5 |
| 原文出处 | 1284 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314676100006 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119168]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yue,Zhang, Jia,Wang, Mengjing,et al. Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings[J]. analytical chemistry,2013,85(3):1280-1284. |
| APA | Yuan, Yue,Zhang, Jia,Wang, Mengjing,Mei, Bin,Guan, Yafeng,&Liang, Gaolin.(2013).Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings.analytical chemistry,85(3),1280-1284. |
| MLA | Yuan, Yue,et al."Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings".analytical chemistry 85.3(2013):1280-1284. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。