An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells
文献类型：期刊论文
| 作者 | Wang, Lei1,2,3; Zhou, Yongjin J.1,2; Ji, Debin1,2; Zhao, Zongbao K.1,2,3 |
| 刊名 | journal of microbiological methods
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 93期号:2页码:73-76 |
| 关键词 | Intracellular aqueous volume NAD(+) Escherichia coil Metabolite Agrobacterium tumefaciens |
| 产权排序 | 待补充 |
| 通讯作者 | 赵宗保 |
| 合作状况 | 英 |
| 英文摘要 | the intracellular aqueous volumes (v-in) of microorganisms are fundamental data that can be used for estimating absolute cellular enzyme and metabolite concentrations. because traditional methods are time-consuming and costly, the v-in data have been largely estimated ambiguously. here we developed an nad(+) concentration-dependent method and demonstrated its usefulness for accurate estimation of the v-in value of escherichia coil cells. the v-in value of e. coli bl21(de3) cells was determined to be 1.9 mu l.mg(-1), which is 17% lower than that of the commonly assumed data. similarly, the v-in value of agrobacterium tumefaciens agl1 cells was determined to be 1.8 mu l.mg(-1). because nad(+) is routinely quantified during metabolite analysis, it may be integrated into metabolomic data collection with little additional time and labor expenditure. this method should also be applicable to estimate the v-in data of other prokaryotic microorganisms. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; microbiology |
| 关键词[WOS] | metabolite concentrations ; water volume ; glucose-6-phosphate-dehydrogenase ; quantification ; metabolomics ; cerevisiae |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 76 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318754600001 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119317]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lei,Zhou, Yongjin J.,Ji, Debin,et al. An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells[J]. journal of microbiological methods,2013,93(2):73-76. |
| APA | Wang, Lei,Zhou, Yongjin J.,Ji, Debin,&Zhao, Zongbao K..(2013).An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells.journal of microbiological methods,93(2),73-76. |
| MLA | Wang, Lei,et al."An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells".journal of microbiological methods 93.2(2013):73-76. |
入库方式： OAI收割
来源：大连化学物理研究所
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