Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity
文献类型：期刊论文
| 作者 | Liu, Jia; Peng, Juan; Shen, Shuai; Jin, Qianru; Li, Can; Yang, Qihua |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 19期号:8页码:2711-2719 |
| 关键词 | biocatalysis immobilization lipase mesoporous materials nanopores |
| 产权排序 | 待补充 |
| 通讯作者 | 李灿 ; 杨启华 |
| 合作状况 | 英 |
| 英文摘要 | macromolecular crowding is an ubiquitous phenomenon in living cells that significantly affects the function of enzymes invivo. however, this effect has not been paid much attention in the research of the immobilization of enzymes onto mesoporous silica. herein, we report the combined effects of macromolecular crowding and surface hydrophobicity on the performance of an immobilized enzyme by accommodating lipase molecules into a series of mesoporous silicas with different amounts of inert poly(methacrylate) (pma) covalently anchored inside the nanopores. the incorporation of the pma polymer into the nanopores of mesoporous silica enables the fabrication of a crowded and hydrophobic microenvironment for the immobilized enzyme and the variation in polymer content facilitates an adjustment of the degree of crowding and surface properties of this environment. based on this system, the catalytic features of immobilized lipase were investigated as a function of polymer content in nanopores and the results indicated that the catalytic efficiency, thermostability, and reusability of immobilized lipase could all be improved by taking advantage of the macromolecular crowding effect and surface hydrophobicity. these findings provide insight into the possible functions of the macromolecular crowding effect, which should be considered and integrated into the fabrication of suitable mesoporous silicas to improve enzyme immobilization. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | protein stability ; interfacial adsorption ; biochemical reactions ; mesoporous materials ; lipase ; confinement ; immobilization ; silica ; biocatalysis ; hybrid |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 2719 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314926300020 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119330]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia,Peng, Juan,Shen, Shuai,et al. Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity[J]. chemistry-a european journal,2013,19(8):2711-2719. |
| APA | Liu, Jia,Peng, Juan,Shen, Shuai,Jin, Qianru,Li, Can,&Yang, Qihua.(2013).Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity.chemistry-a european journal,19(8),2711-2719. |
| MLA | Liu, Jia,et al."Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity".chemistry-a european journal 19.8(2013):2711-2719. |
入库方式： OAI收割
来源：大连化学物理研究所
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