Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins
文献类型：期刊论文
| 作者 | Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 405期号:15页码:5321-5331 |
| 关键词 | Click chemistry Boronate affinity hybrid monolith Glycoproteins Enrichment Egg sample |
| 产权排序 | 待补充 |
| 通讯作者 | 陈朗星 ; 张玉奎 |
| 合作状况 | 英 |
| 英文摘要 | a novel strategy for preparation of a boronate affinity hybrid monolith was developed using a cu(i)-catalyzed 1,3-dipolar azide-alkyne cycloaddition (cuaac) reaction of an alkyne-boronate ligand with an azide-functionalized monolithic intermediate. an azide-functionalized hybrid monolith was first synthesized via a single-step procedure to provide reactive sites for click chemistry; then the alkyne-boronate ligands were covalently immobilized on the azide-functionalized hybrid monolith via an in-column cuaac reaction to form a boronate affinity hybrid monolith under mild conditions. the boronate affinity monolith was characterized and evaluated by means of elemental analysis, fourier transform infrared spectroscopy, and scanning electron microscopy. the boronate affinity hybrid monolith exhibited excellent specificity toward nucleosides and glycoproteins, which were chosen as test cis-diol-containing compounds under neutral conditions. the binding capacity of the monolith for the glycoprotein ovalbumin was 2.36 mg center dot g(-1) at ph 7.0. the practicability of the boronate affinity hybrid monolithic material was demonstrated by specific capture of the glycoproteins ovalbumin and ovotransferrin from an egg sample. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | click chemistry ; capillary chromatography ; liquid-chromatography ; selective enrichment ; efficient strategy ; mass-spectrometry ; stationary phases ; cis-diol ; column ; separation |
| 收录类别 | SCI |
| 资助信息 | 2，5 |
| 原文出处 | 5331 |
| 语种 | 英语 |
| WOS记录号 | WOS:000319168500029 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119341]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, F.,Mao, J.,He, X. W.,et al. Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins[J]. analytical and bioanalytical chemistry,2013,405(15):5321-5331. |
| APA | Yang, F.,Mao, J.,He, X. W.,Chen, L. X.,&Zhang, Y. K..(2013).Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins.analytical and bioanalytical chemistry,405(15),5321-5331. |
| MLA | Yang, F.,et al."Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins".analytical and bioanalytical chemistry 405.15(2013):5321-5331. |
入库方式： OAI收割
来源：大连化学物理研究所
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