中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

文献类型:期刊论文

作者Gao, Feng-Xian1,2; Ma, Xiao-Tong1,2; He, Xi-Wen1,2; Li, Wen-You1,2; Zhang, Yu-Kui1,2,3
刊名colloids and surfaces a-physicochemical and engineering aspects
出版日期2013-09-20
卷号433期号:20页码:191-199
关键词Molecular imprinting Surface imprinting Covalent immobilization Nanosphere Ovalbumin
产权排序待补充
通讯作者李文友
合作状况
英文摘要through covalent immobilization of template and surface imprinting, smart molecularly imprinted polymer nanospheres were developed for selective separation of the glycoprotein ovalbumin (ob). first, a boronic acid group-bearing poly (methyl methacrylate) (b-pmma) nanosphere was synthesized directly at the high temperature of 70 degrees c. the b-pmma nanosphere could pre-immobilize the template ob on its surface by forming reversible covalent bonds. then the precipitation polymerization of n-isopropylacrylamide (nipaam) and acrylamide (aam) readily occurred on the b-pmma nanosphere as a core at room temperature, leading to the formation of core-shell molecular imprinting nanosphere. the experiments showed the rebinding affinity of the imprinted nanospheres was thermo and ph-dependent. the resulting imprinted nanospheres showed high adsorption capacity and good specific recognition behavior toward the template molecule, and no obvious reusability deterioration was observed. most notably, the imprinted nanospheres reached saturated adsorption within 20 min, indicating faster rebinding kinetics. in addition, the imprinted nanospheres were successfully applied to selectively separate the target ob from an egg white sample. (c) 2013 elsevier b.v. all rights reserved.
WOS标题词science & technology ; physical sciences
学科主题物理化学
类目[WOS]chemistry, physical
研究领域[WOS]chemistry
关键词[WOS]protein recognition ; mesoporous silica ; nanoparticles ; glycopeptides ; enrichment ; hydrogels ; template ; capture ; chromatography ; antibodies
收录类别SCI
资助信息2,5
原文出处199
语种英语
WOS记录号WOS:000321538100023
公开日期2014-09-11
源URL[http://159.226.238.44/handle/321008/119454]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,et al. Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein[J]. colloids and surfaces a-physicochemical and engineering aspects,2013,433(20):191-199.
APA Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2013).Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein.colloids and surfaces a-physicochemical and engineering aspects,433(20),191-199.
MLA Gao, Feng-Xian,et al."Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein".colloids and surfaces a-physicochemical and engineering aspects 433.20(2013):191-199.

入库方式: OAI收割

来源:大连化学物理研究所

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