Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein
文献类型：期刊论文
| 作者 | Gao, Feng-Xian1,2; Ma, Xiao-Tong1,2; He, Xi-Wen1,2; Li, Wen-You1,2; Zhang, Yu-Kui1,2,3 |
| 刊名 | colloids and surfaces a-physicochemical and engineering aspects
 |
| 出版日期 | 2013-09-20 |
| 卷号 | 433期号:20页码:191-199 |
| 关键词 | Molecular imprinting Surface imprinting Covalent immobilization Nanosphere Ovalbumin |
| 产权排序 | 待补充 |
| 通讯作者 | 李文友 |
| 合作状况 | 英 |
| 英文摘要 | through covalent immobilization of template and surface imprinting, smart molecularly imprinted polymer nanospheres were developed for selective separation of the glycoprotein ovalbumin (ob). first, a boronic acid group-bearing poly (methyl methacrylate) (b-pmma) nanosphere was synthesized directly at the high temperature of 70 degrees c. the b-pmma nanosphere could pre-immobilize the template ob on its surface by forming reversible covalent bonds. then the precipitation polymerization of n-isopropylacrylamide (nipaam) and acrylamide (aam) readily occurred on the b-pmma nanosphere as a core at room temperature, leading to the formation of core-shell molecular imprinting nanosphere. the experiments showed the rebinding affinity of the imprinted nanospheres was thermo and ph-dependent. the resulting imprinted nanospheres showed high adsorption capacity and good specific recognition behavior toward the template molecule, and no obvious reusability deterioration was observed. most notably, the imprinted nanospheres reached saturated adsorption within 20 min, indicating faster rebinding kinetics. in addition, the imprinted nanospheres were successfully applied to selectively separate the target ob from an egg white sample. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, physical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | protein recognition ; mesoporous silica ; nanoparticles ; glycopeptides ; enrichment ; hydrogels ; template ; capture ; chromatography ; antibodies |
| 收录类别 | SCI |
| 资助信息 | 2，5 |
| 原文出处 | 199 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321538100023 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119454]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,et al. Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein[J]. colloids and surfaces a-physicochemical and engineering aspects,2013,433(20):191-199. |
| APA | Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2013).Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein.colloids and surfaces a-physicochemical and engineering aspects,433(20),191-199. |
| MLA | Gao, Feng-Xian,et al."Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein".colloids and surfaces a-physicochemical and engineering aspects 433.20(2013):191-199. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。