Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites
文献类型：期刊论文
| 作者 | Li, Hua; Shan, Yuanhong; Qiao, Lizhen; Dou, Abo; Shi, Xianzhe; Xu, Guowang |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013-12-03 |
| 卷号 | 85期号:23页码:11585-11592 |
| 产权排序 | 待补充 |
| 通讯作者 | 石先哲 ; 许国旺 |
| 合作状况 | 英 |
| 英文摘要 | ribosylated metabolites, especially modified nucleosides, have been extensively evaluated as cancer-related biomarkers. boronate adsorbents are considered to be promising materials for extracting them from complex matrices. however, the enrichment of ribosylated metabolites in low abundance is still a challenge due to the limited capacity and selectivity of the existing boronate adsorbents. in this study, a novel type of magnetic nanoparticles named fe3o4@sio2@pei-fpba was synthesized by grafting polyethyleneimine (pei) onto the surface of fe3o4@sio2 before modification by boronate groups. the high density of the amino groups on the pei chains supplied a large number of binding sites for boronate groups. thus, the adsorption capacity (1.34 +/- 0.024 mg/g) of the nanoparticles, which is 6- to 7-fold higher than that of analogous materials, was greatly improved. the unreacted secondary amines and tertiary amines of the pei enhanced the aqueous solubility of the nanoparticles, which could efficiently reduce nonspecific adsorption. the nanoparticles were able to capture 1,2 cis-diol nucleosides from 1000-fold interferences. moreover, the flexible chains of pei were favorable for effective enrichment and quick equilibration (<2 min). finally, 60 ribose conjugates were enriched from human urine using the nanoparticles. among them, 43 were identified to be nucleosides and other ribosylated metabolites. nine low abundance modified nucleosides were detected for the first time. in conclusion, fe3o4@sio2@pei-fpba is an attractive candidate material for the highly selective enrichment of 1,2-cis-diol compounds. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; performance liquid-chromatography ; functionalized mesoporous silica ; potential tumor-markers ; urinary nucleosides ; breast-cancer ; ms analysis ; acid ; quantification ; identification |
| 收录类别 | SCI |
| 资助信息 | 1，1 |
| 原文出处 | 11592 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327999800058 |
| 公开日期 | 2014-09-11 |
| 源URL | [http://159.226.238.44/handle/321008/119480]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hua,Shan, Yuanhong,Qiao, Lizhen,et al. Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites[J]. analytical chemistry,2013,85(23):11585-11592. |
| APA | Li, Hua,Shan, Yuanhong,Qiao, Lizhen,Dou, Abo,Shi, Xianzhe,&Xu, Guowang.(2013).Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites.analytical chemistry,85(23),11585-11592. |
| MLA | Li, Hua,et al."Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites".analytical chemistry 85.23(2013):11585-11592. |
入库方式： OAI收割
来源：大连化学物理研究所
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